P2Y6 regulates cytoskeleton reorganization and cell migration of C2C12 myoblasts via ROCK pathway
| العنوان: | P2Y6 regulates cytoskeleton reorganization and cell migration of C2C12 myoblasts via ROCK pathway |
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| المؤلفون: | Bimeng Zhang, Gao Yingna, Xianmin Song, Mengjie Chen, Donghui Chen, Wei Wang, Hongliang Zheng, Kaiyong Zhang |
| المصدر: | Journal of Cellular Biochemistry. 119:1889-1898 |
| بيانات النشر: | Wiley, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | rho GTP-Binding Proteins, 0301 basic medicine, RHOA, Biology, Biochemistry, Uridine Diphosphate, Cell Line, Myoblasts, Mice, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, Isothiocyanates, medicine, Animals, Myocyte, Cytoskeleton, Molecular Biology, Actin, rho-Associated Kinases, Receptors, Purinergic P2, Thiourea, Skeletal muscle, Cell migration, Cell Biology, Cofilin, musculoskeletal system, Cell biology, 030104 developmental biology, medicine.anatomical_structure, biology.protein, Calcium, rhoA GTP-Binding Protein, tissues, C2C12, 030217 neurology & neurosurgery, Signal Transduction |
| الوصف: | Migration of skeletal muscle precursor cells is required for limb muscle development and skeletal muscle repair. This study aimed to examine the role of P2Y6 receptor in C2C12 myoblasts migration. C2C12 myoblasts were treated with P2Y6 agonist UDP, P2Y6 antagonist MRS2578, Ca2+ channel blocker BTP2, or ROCK inhibitor GSK269962 or Y27632, and the migration ability of C2C12 cells was assessed by wound healing assay. The cellular Ca2+ content was analyzed with fluo-4 probe and the activation of ROCK (phosphorlyation of LIMK and cofilin) was assayed by western blot. The cytoskeleton was labeled with Actin-Tracker Green and Tubulin-Tracker-Red. Silencing P2Y6 expression in C2C12 myoblasts reduced intracellular Ca2+ content and cell motility. Whereas UDP increased cellular Ca2+ content, actin filaments, and cell migration, MRS2578 had the opposite effects. The effects of UDP were abrogated by BTP2 and GSK269962 (and Y27632). Disruption of P2Y6 signaling pathway caused C2C12 myoblasts to have an elongated morphology. These results demonstrated that P2Y6 signaled through Ca2+ influx and RhoA/ROCK to reorganize cytoskeleton and promote migration in myoblasts. |
| تدمد: | 1097-4644 0730-2312 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74e68d343e7f38d00c287ffe5ab5ffd9Test https://doi.org/10.1002/jcb.26350Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....74e68d343e7f38d00c287ffe5ab5ffd9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974644 07302312 |
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