Porcine Bladder Replacement with a Bilayer Silk Fibroin Enhanced Prosthetic Reservoir: A Feasibility Study
| العنوان: | Porcine Bladder Replacement with a Bilayer Silk Fibroin Enhanced Prosthetic Reservoir: A Feasibility Study |
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| المؤلفون: | Pengbo Jiang, Sohrab N. Ali, Raphael B. Arada, Akhil Peta, Andrew Brevik, Maged Ayad, Andrew Shin, Kalon L. Morgan, Krista Larson, Erik Larson, Gokhan Gundogdu, Shlomi Tapiero, Ted Farzaneh, Roshan M. Patel, Joshua Mauney, Jaime Landman, Ralph V. Clayman |
| المصدر: | Journal of Endourology. 37:341-352 |
| بيانات النشر: | Mary Ann Liebert Inc, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Urology |
| الوصف: | The creation of synthetic reservoirs for bladder replacement has been limited by challenges of interfacing synthetic materials and native tissue. We sought to overcome this challenge by utilizing a novel bilayer silk fibroin scaffold (BLFS) as an intermediary towards the development of an acellular prosthetic reservoir.Under institutionally-approved protocols, 3D-printed reservoirs were implanted in six juvenile female pigs after cystectomy. BLFS was attached to the in-situ prosthetic reservoir serving as an intermediary to native ureteral and urethral tissue anastomoses. Our first protocol allowed four pigs to be survived up to seven days, and the second protocol allowed two pigs to be survived for up to one year. At the first sign of functional decline or the end of the study period, the animals were euthanized, and kidneys, ureters, prosthetic bladder, and urethra were harvested en-bloc for histopathology.The first two pigs had anastomotic urine leaks due to design flaws resulting in early termination. The third pig had acute renal failure resulting in early termination. The artificial bladder design was modified in subsequent iterations. The fourth pig survived for seven days and upon autopsy, had intact urethral and ureteral anastomoses. The fifth and sixth pigs survived for 11 and 12 weeks, respectively, before they were sacrificed due to failure to thrive. One animal developed an enteric fistula. The other animal had an intact anastomosis, and the BLFS was identified at the ureteral and urethral anastomoses on histopathogic analysis.Replacing the porcine bladder with a prosthetic bladder was successfully achieved for up to three months, the second longest survival period for a nonbiological bladder alternative. Bilayer silk fibroin scaffold was used for the first time to create an interface between synthetic material and biologic tissue by allowing ingrowth of urothelium onto the acellular alloplastic bladder. |
| تدمد: | 1557-900X 0892-7790 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::737e952c4cc9ab789afd52499a653319Test https://doi.org/10.1089/end.2022.0296Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....737e952c4cc9ab789afd52499a653319 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1557900X 08927790 |
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