Cell lineage specificity in G-CSF receptor gene methylation
| العنوان: | Cell lineage specificity in G-CSF receptor gene methylation |
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| المؤلفون: | Sven Olaf Frahm, Klaus Heidorn, D Körbächer, J. Felgner, Reza Parwaresch |
| المصدر: | Leukemia. 13:530-534 |
| بيانات النشر: | Springer Science and Business Media LLC, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Cancer Research, Transcription, Genetic, Cellular differentiation, Biology, Leukemia, Myelomonocytic, Acute, Monocytes, Cell Line, Growth Factor Receptor Gene, Cell surface receptor, Macrophages, Alveolar, Gene expression, Tumor Cells, Cultured, Humans, Cell Lineage, Lymphocytes, Receptor, Cell Line, Transformed, Gene Expression Regulation, Leukemic, Macrophages, Gene Expression Regulation, Developmental, Cell Differentiation, Promoter, U937 Cells, Hematology, Methylation, DNA Methylation, Hematopoiesis, Neoplasm Proteins, Oncology, Receptors, Granulocyte Colony-Stimulating Factor, DNA methylation, Leukocytes, Mononuclear, Macrophages, Peritoneal, Neoplastic Stem Cells, Cancer research, K562 Cells, Polymorphism, Restriction Fragment Length |
| الوصف: | In hematopoiesis the evolution of specialized cell lineages from a common stem cell is mediated by lineage-specific growth factors. The role of DNA methylation in the multilevel regulation of the differential gene expression, especially in the case of growth factor receptor genes, has remained elusive. In earlier studies we showed a lineage-specific methylation pattern of the M-CSF receptor gene c-fms in blood monocytes and tissue macrophages. Here, we provide evidence that a lineage- specific hypomethylation exists for the G-CSF receptor gene for myelomonocytic cells but not in lymphocytes without any interindividual differences. Constant differences were found between alveolar and peritoneal macrophages with a lesser degree of methylation in peritoneal macrophages. Acute myelomonocytic leukemias showed an increased methylation as compared with normal granulocytes and monocytes. All permanent cell lines analyzed revealed hypermethylation of the G-CSF receptor gene. Lymphocytes of B-CLL showed a strong hypermethylation of this gene. Increased methylation has been shown to be inversely correlated with transcriptional gene activities. We conclude that the methylation pattern of growth factor receptor genes may be one of the regulatory mechanisms in multi-lineage differentiation. |
| تدمد: | 1476-5551 0887-6924 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::737be11ff47b9c70b6cf2189700c205fTest https://doi.org/10.1038/sj.leu.2401386Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....737be11ff47b9c70b6cf2189700c205f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765551 08876924 |
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