Adipose tissue, but not skeletal muscle, sirtuin 1 expression is decreased in obesity and related to insulin sensitivity
| العنوان: | Adipose tissue, but not skeletal muscle, sirtuin 1 expression is decreased in obesity and related to insulin sensitivity |
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| المؤلفون: | Natalia Matulewicz, Magdalena Stefanowicz, Monika Karczewska-Kupczewska, Agnieszka Nikolajuk |
| المصدر: | Endocrine |
| بيانات النشر: | Springer US, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Adipose tissue, Skeletal muscle, Fatty Acids, Nonesterified, 0302 clinical medicine, Endocrinology, Sirtuin 1, Hyperinsulinemia, Phospholipids, Glucose Transporter Type 4, biology, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, 3. Good health, medicine.anatomical_structure, Original Article, Emulsions, Adiponectin, Adult, medicine.medical_specialty, Adolescent, 030209 endocrinology & metabolism, 03 medical and health sciences, Young Adult, Insulin resistance, SIRT1, Internal medicine, Hyperinsulinism, medicine, Humans, Obesity, Muscle, Skeletal, business.industry, Heparin, Insulin, Body Weight, medicine.disease, Soybean Oil, 030104 developmental biology, biology.protein, business |
| الوصف: | Purpose Sirtuin 1 may regulate glucose and lipid metabolism. We aimed to assess adipose tissue and skeletal muscle sirtuin 1 expression in relation to insulin sensitivity, the expression of proinflammatory and metabolic genes, and to study the regulation of sirtuin 1 expression by hyperinsulinemia and circulating free fatty acids elevation. Methods We examined 60 normal-weight, 42 overweight and 15 obese young subjects. The hyperinsulinemic-euglycemic clamp technique was applied throughout to measure insulin sensitivity. In 20 subjects, two 6 h clamps were performed, one of them with concurrent Intralipid/heparin infusion. Biopsies of subcutaneous adipose tissue and skeletal muscle were collected for the measurement of gene and protein expression. Results Obese subjects had lower adipose sirtuin 1 in comparison with normal-weight and overweight participants. Muscle sirtuin 1 did not differ between the groups. Adipose tissue sirtuin 1 was related to insulin sensitivity, adipose tissue SLC2A4. The relationship between adipose tissue sirtuin 1 and insulin sensitivity was still present after controlling for BMI, however, it disappeared after controlling for adipose tissue SLC2A4. Muscle sirtuin 1 was not related to insulin sensitivity. Hyperisulinemia decreased adipose tissue and increased muscle sirtuin 1 expression. Intralipid/heparin infusion negated these effects. Conclusions Adipose tissue, but not muscle, sirtuin 1 is associated with insulin sensitivity in humans, possibly because of its correlation with adipose tissue SLC2A4 expression. Insulin differentially regulates adipose tissue and skeletal muscle sirtuin 1 expression in the short-term and circulating free fatty acids elevation negates these effects, which may be associated with lipid-induced insulin resistance. |
| اللغة: | English |
| تدمد: | 1559-0100 1355-008X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73333e46e26f9f402e847256f241a4e5Test http://europepmc.org/articles/PMC5893655Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....73333e46e26f9f402e847256f241a4e5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15590100 1355008X |
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