Cell interactions influencing murine marrow megakaryocytes: nature of the potentiator cell in bone marrow
| العنوان: | Cell interactions influencing murine marrow megakaryocytes: nature of the potentiator cell in bone marrow |
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| المؤلفون: | Peter Ralph, Ilona Nakoinz, Heather Jackson, Neil Williams |
| المصدر: | Blood. 57:157-163 |
| بيانات النشر: | American Society of Hematology, 1981. |
| سنة النشر: | 1981 |
| مصطلحات موضوعية: | C57BL/6, Cell, Immunology, Stimulation, Bone Marrow Cells, Cell Communication, Biochemistry, Bone and Bones, Cell Line, Mice, Megakaryocyte, Colony-Stimulating Factors, Bone Marrow, medicine, Cell Adhesion, Animals, Progenitor cell, Lung, Mice, Inbred C3H, biology, Macrophages, Cell Biology, Hematology, Potentiator, biology.organism_classification, Cell biology, Mice, Inbred C57BL, medicine.anatomical_structure, Myeloid-derived Suppressor Cell, Bone marrow, Mitogens, Megakaryocytes |
| الوصف: | Auxiliary bone marrow cells are required for optimal murine megakaryocyte colony formation in addition to progenitor cells and a colony stimulating activity (CSA) present in WEHI-3 cell conditioned medium. These auxiliary cells are adherent, with a sedimentation rate of 5.8 mm hr-1 and buoyant density of 1.065–1.078 gcm-3. The activity from bone marrow cells is loss at irradiation doses above 900 rad. Bone marrow cells with these characteristics, and supernatants from lung, bone shafts, and peritoneal exudate cells were all active in enhancing megakaryocyte colony incidences in mouse bone marrow cultures above those stimulated by an obligatory activity in WEHI-3 cell conditioned medium. Certain macrophage cell lines (J774, P388D1) could elaborate the activity. This study confirms that a potentiation activity enhances CSA stimulation of megakaryocyte colony formation. The potentiator is elaborated by bone marrow cells in limiting amounts requiring either high cell concentrations or an exogenous source of the activity for optimal colony growth. |
| تدمد: | 1528-0020 0006-4971 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::705f9ad9460d0193fa134f411d1791a0Test https://doi.org/10.1182/blood.v57.1.157.bloodjournal571157Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....705f9ad9460d0193fa134f411d1791a0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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