Comparison of three different application routes of butyrate to improve colonic anastomotic strength in rats
العنوان: | Comparison of three different application routes of butyrate to improve colonic anastomotic strength in rats |
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المؤلفون: | Audrey C. H. M. Jongen, S. van Rijn, Nicole D. Bouvy, Joanna W. A. M. Bosmans, Bas Boonen, Marion J.J. Gijbels, Luca Stucchi, Eleonora Marsich, Francesca Scognamiglio |
المساهمون: | RS: NUTRIM - R1 - Metabolic Syndrome, Promovendi NTM, Surgery, RS: NUTRIM - R2 - Liver and digestive health, Ondersteunend personeel NTM, Pathologie, Moleculaire Genetica, RS: CARIM - R3.06 - The vulnerable plaque: makers and markers, MUMC+: MA Heelkunde (9), Bosmans, J. W. A. M., Jongen, A. C. H. M., Boonen, B. T. C., van Rijn, S., Scognamiglio, Francesca, Stucchi, L., Gijbels, M. J. J., Marsich, Eleonora, Bouvy, N. D. |
المصدر: | International Journal of Colorectal Disease, 32(3), 305-313. Springer, Cham International Journal of Colorectal Disease |
بيانات النشر: | Springer, Cham, 2017. |
سنة النشر: | 2017 |
مصطلحات موضوعية: | Anastomoses, Animal study, medicine.medical_specialty, Colon, SURGERY, Anastomotic healing, Anastomotic Leak, Butyrate, Anastomosis, Real-Time Polymerase Chain Reaction, Gastroenterology, DISEASE, 03 medical and health sciences, 0302 clinical medicine, LEAKAGE, INFLAMMATION, Internal medicine, medicine, Pressure, Animals, Colorectal anastomotic leakage, Rats, Wistar, TRIBUTYRIN, ARTICLE, CANCER CELLS, business.industry, Drug Administration Routes, MORTALITY, Anastomosis, Surgical, HYALURONIC-ACID, Colorectal surgery, Matrix Metalloproteinases, 3. Good health, PRODRUG, Gene Expression Regulation, Anastomotic leakage, 030220 oncology & carcinogenesis, Butyric Acid, 030211 gastroenterology & hepatology, Original Article, Collagen, business, Anastomose |
الوصف: | Introduction Despite extensive research, anastomotic leakage (AL) remains one of the most dreaded complications after colorectal surgery. Since butyrate enemas are known to enhance anastomotic healing, several administration routes have been explored in this study. Methods Three intraluminal approaches involving butyrate were investigated: (1) butyrin-elucidating patch, (2) a single injection of hyaluronan-butyrate (HA-But) prior to construction of the proximal anastomosis and (3) rectal hyaluronan-butyrate (HA-But) enemas designed for distal anastomoses. The main outcome was AL and secondary outcomes were bursting pressure, histological analysis of the anastomosis, zymography to detect MMP activity and qPCR for gene expression of MMP2, MMP9, MUC2 and TFF3. Results Neither the patches nor the injections led to a reduction of AL in experiments 1 and 2. In experiment 3, a significant reduction of AL was accomplished with the (HA-But) enema compared to the control group together with a higher bursting pressure. Histological analysis detected only an increased inflammation in experiment 2 in the hyaluronan injection group compared to the control group. No other differences were found regarding wound healing. Zymography identified a decreased proenzyme of MMP9 when HA-But was administered as a rectal enema. qPCR did not show any significant differences between groups in any experiment. Conclusion Butyrate enemas are effective in the enhancement of colonic anastomosis. Enhanced butyrate-based approaches designed to reduce AL in animal models for both proximal and distal anastomoses were not more effective than were butyrate enemas alone. Further research should focus on how exogenous butyrate can improve anastomotic healing after gastrointestinal surgery. Electronic supplementary material The online version of this article (doi:10.1007/s00384-016-2718-z) contains supplementary material, which is available to authorized users. |
وصف الملف: | ELETTRONICO |
اللغة: | English |
تدمد: | 1432-1262 0179-1958 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ef0d7b05e24cfbb69bf589b479a6feeTest https://cris.maastrichtuniversity.nl/en/publications/c42e0dd7-ce41-4db3-b69a-871eb54b4e51Test |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....6ef0d7b05e24cfbb69bf589b479a6fee |
قاعدة البيانات: | OpenAIRE |
تدمد: | 14321262 01791958 |
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