Mitochondrial gene replacement in primate offspring and embryonic stem cells
| العنوان: | Mitochondrial gene replacement in primate offspring and embryonic stem cells |
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| المؤلفون: | Hathaitip Sritanaudomchai, Joy Woodward, Shoukhrat Mitalipov, Lisa Clepper, Ying Li, Hong Ma, Michelle Sparman, Olena Kolotushkina, Masahito Tachibana, Cathy Ramsey |
| المصدر: | Nature |
| بيانات النشر: | Springer Science and Business Media LLC, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Male, Mitochondrial DNA, Mitochondrial Diseases, Reproductive Techniques, Assisted, Mitochondrial replacement therapy, Fertilization in Vitro, Biology, DNA, Mitochondrial, Genome, Article, Paternal mtDNA transmission, Pregnancy, Spindle transfer, Animals, Embryonic Stem Cells, Cell Nucleus, Genetics, Multidisciplinary, Genetic transfer, Embryo, Embryo Transfer, Macaca mulatta, Nuclear DNA, Meiosis, Genes, Mitochondrial, Genome, Mitochondrial, Mutation, Oocytes, Female |
| الوصف: | Mitochondria are found in all eukaryotic cells and contain their own genome (mitochondrial DNA or mtDNA). Unlike the nuclear genome, which is derived from both the egg and sperm at fertilization, the mtDNA in the embryo is derived almost exclusively from the egg; that is, it is of maternal origin. Mutations in mtDNA contribute to a diverse range of currently incurable human diseases and disorders. To establish preclinical models for new therapeutic approaches, we demonstrate here that the mitochondrial genome can be efficiently replaced in mature non-human primate oocytes (Macaca mulatta) by spindle-chromosomal complex transfer from one egg to an enucleated, mitochondrial-replete egg. The reconstructed oocytes with the mitochondrial replacement were capable of supporting normal fertilization, embryo development and produced healthy offspring. Genetic analysis confirmed that nuclear DNA in the three infants born so far originated from the spindle donors whereas mtDNA came from the cytoplast donors. No contribution of spindle donor mtDNA was detected in offspring. Spindle replacement is shown here as an efficient protocol replacing the full complement of mitochondria in newly generated embryonic stem cell lines. This approach may offer a reproductive option to prevent mtDNA disease transmission in affected families. |
| تدمد: | 1476-4687 0028-0836 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e466a88d1dd953dea57bf49e8f2ff85Test https://doi.org/10.1038/nature08368Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6e466a88d1dd953dea57bf49e8f2ff85 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14764687 00280836 |
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