Comparability of imazapyr-resistant Arabidopsis created by transgenesis and mutagenesis
| العنوان: | Comparability of imazapyr-resistant Arabidopsis created by transgenesis and mutagenesis |
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| المؤلفون: | Yuzuki Manabe, Hélène Labbé, Brian Miki, Jaimie Schnell, Nik Kovinich |
| المصدر: | Transgenic Research. 21:1255-1264 |
| بيانات النشر: | Springer Science and Business Media LLC, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Transgene, Mutant, Arabidopsis, Mutagenesis (molecular biology technique), Genes, Plant, Niacin, Transcriptome, chemistry.chemical_compound, Gene Expression Regulation, Plant, Genetics, Gene, Oligonucleotide Array Sequence Analysis, Acetolactate synthase, biology, Arabidopsis Proteins, Gene Expression Profiling, Genetic Complementation Test, Imidazoles, Imazapyr, Plants, Genetically Modified, biology.organism_classification, chemistry, Mutagenesis, Mutation, biology.protein, Animal Science and Zoology, Agronomy and Crop Science, Herbicide Resistance, Biotechnology |
| الوصف: | The Arabidopsis CSR1 gene codes for the enzyme acetohydroxyacid synthase (AHAS, EC 2.2.1.6), also known as acetolactate synthase, which catalyzes the first step in branched-chain amino acid biosynthesis. It is inhibited by several classes of herbicides, including the imidazolinone herbicides, such as imazapyr; however, a substitution mutation in csr1-2 (Ser-653-Asn) confers selective resistance to the imidazolinones. The transcriptome of csr1-2 seedlings grown in the presence of imazapyr has been shown in a previous study (Manabe in Plant Cell Physiol 48:1340-1358, 2007) to be identical to that of wild-type seedlings indicating that AHAS is the sole target of imazapyr and that the mutation is not associated with pleiotropic effects detectable by transcriptome analysis. In this study, a lethal null mutant, csr1-7, created by a T-DNA insertion into the CSR1 gene was complemented with a randomly-inserted 35S/CSR1-2/NOS transgene in a subsequent genetic transformation event. A comparison of the csr1-2 substitution mutant with the transgenic lines revealed that all were resistant to imazapyr; however, the transgenic lines yielded significantly higher levels of resistance and greater biomass accumulation in the presence of imazapyr. Microarray analysis revealed few differences in their transcriptomes. The most notable was a sevenfold to tenfold elevation in the CSR1-2 transcript level. The data indicate that transgenesis did not create significant unintended pleiotropic effects on gene expression and that the mutant and transgenic lines were highly similar, except for the level of herbicide resistance. |
| تدمد: | 1573-9368 0962-8819 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dc1a25b6961962f15bc6a80de300defTest https://doi.org/10.1007/s11248-012-9597-zTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....6dc1a25b6961962f15bc6a80de300def |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15739368 09628819 |
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