The Reproducibility of the Immunohistochemical PD-L1 Testing in Non-Small-Cell Lung Cancer: A Multicentric Italian Experience
| العنوان: | The Reproducibility of the Immunohistochemical PD-L1 Testing in Non-Small-Cell Lung Cancer: A Multicentric Italian Experience |
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| المؤلفون: | Diego Cortinovis, Emanuele Valtorta, Nicola Fusco, Fabio Pagni, Umberto Malapelle, Patrizia Morbini, Alexiadis Spyridon, Antonino Iaccarino, Manuela Bimbatti, Francesca Bono, Chiara Piva, Gabriella Gaudioso, Elena Vigliar, Mario Zocchi |
| المساهمون: | Vigliar, E, Malapelle, U, Bono, F, Fusco, N, Cortinovis, D, Valtorta, E, Spyridon, A, Bimbatti, M, Zocchi, M, Piva, C, Gaudioso, G, Iaccarino, A, Morbini, P, Pagni, F, Vigliar, E., Malapelle, U., Bono, F., Fusco, N., Cortinovis, D., Valtorta, E., Spyridon, A., Bimbatti, M., Zocchi, M., Piva, C., Gaudioso, G., Iaccarino, A., Morbini, P., Pagni, F. |
| المصدر: | BioMed Research International BioMed Research International, Vol 2019 (2019) |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Oncology, Adult, medicine.medical_specialty, Lung Neoplasms, Article Subject, Programmed Cell Death 1 Receptor, lcsh:Medicine, Pembrolizumab, Antibodies, Monoclonal, Humanized, General Biochemistry, Genetics and Molecular Biology, B7-H1 Antigen, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, PD-L1, Carcinoma, Non-Small-Cell Lung, Carcinoma, Biomarkers, Tumor, Medicine, Humans, PD-L1- cancer, Lung cancer, Lung, Retrospective Studies, Reproducibility, General Immunology and Microbiology, biology, business.industry, Macrophages, lcsh:R, Retrospective cohort study, General Medicine, medicine.disease, Immunohistochemistry, 030104 developmental biology, Italy, 030220 oncology & carcinogenesis, Monoclonal, biology.protein, Carcinoma, Squamous Cell, business, Research Article |
| الوصف: | An important harmonization effort was produced by the scientific community to standardize both the preanalytical and interpretative phases of programmed death-ligand 1 (PD-L1) immunohistochemical (IHC) testing in non-small-cell lung cancer (NSCLC). This analysis is crucial for the selection of patients with advanced-stage tumors eligible for treatment with pembrolizumab and potentially with other anti-PD-1/PD-L1 checkpoint inhibitors. This multicentric retrospective study evaluated the reproducibility of PD-L1 testing in the Italian scenario both for closed and open platforms. In the evaluation of the well-known gold-standard combinations (Agilent 22C3 PharmDx on Dako Autostainer versus Roche’s Ventana SP263 on BenchMark), the results confirmed the literature data and showed complete overlapping between the two methods. With regard to the performances by using open platforms, the combination of 22C3 with Dako Omnis or Benchmark obtained good results basically, while the 28,8 clone seemed to be associated with worse scores. |
| وصف الملف: | text/xhtml |
| تدمد: | 2314-6141 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c7bb0d3cd3337137870e47b9cf1b740Test https://pubmed.ncbi.nlm.nih.gov/31111063Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6c7bb0d3cd3337137870e47b9cf1b740 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 |
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