Genome‐wide analysis of odorant‐binding proteins and chemosensory proteins in the sweet potato whitefly, Bemisia tabaci
| العنوان: | Genome‐wide analysis of odorant‐binding proteins and chemosensory proteins in the sweet potato whitefly, Bemisia tabaci |
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| المؤلفون: | Wen Xie, Shaoli Wang, Yuting Yang, Qingjun Wu, Youjun Zhang, Yang Zeng |
| المصدر: | Insect Science |
| بيانات النشر: | Wiley, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, Nymph, 0106 biological sciences, 0301 basic medicine, Odorant binding, Genome, Insect, Genome wide analysis, OBPs, Whitefly, Receptors, Odorant, Bemisia tabaci, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, law.invention, Hemiptera, 03 medical and health sciences, law, Animals, Gene, Phylogeny, Ecology, Evolution, Behavior and Systematics, Polymerase chain reaction, Ovum, Genetics, Phylogenetic tree, biology, Host (biology), Original Articles, biology.organism_classification, CSPs, Gene expression profiling, 010602 entomology, 030104 developmental biology, expression patterns, Insect Science, genome‐wide identification, Insect Proteins, Original Article, Female, phylogenetic, Agronomy and Crop Science |
| الوصف: | Odorant‐binding proteins (OBPs) and chemosensory proteins (CSPs) of insects are thought to play roles in olfactory recognition affecting host choice, copulation, reproduction and other behaviors. Previous descriptions of OBPs and CSPs in the whitefly Bemisia tabaci often provided no or incomplete genetic information. In this study, we present a genome‐wide and transcriptome‐wide investigation of the OBPs and CSPs in B. tabaci MEAM1 (Middle East‐Asia Minor1 species). Eight OBP and 19 CSP genes were identified that covered all previous sequences. Phylogenetic analyses showed that the CSP genes had a lineage‐specific expansion (BtabBCSP1, BtabBCSP3, BtabBCSP13, BtabBCSP17, BtabBCSP18 and BtabBCSP19). Expression profiling of OBPs and CSPs by transcriptome sequencing and quantitative real‐time polymerase chain reaction (qPCR) revealed that expression patterns differed among developmental stages of B. tabaci MEAM1. Five OBP genes and 11 CSP genes significantly differed between males and females; four of the 19 CSP genes were highly expressed in adults, while two were highly expressed in nymphs. The expression profiles of the OBP and CSP genes in different tissues of B. tabaci MEAM1 adults were analyzed by qPCR. Four OBP genes found in B. tabaci MEAM1 were highly expressed in the head. Conversely, only two CSPs were enriched in the head, while the other six CSPs were specifically expressed in other tissues. Our results provide a foundation for future research on OBPs and CSPs in B. tabaci. |
| تدمد: | 1744-7917 1672-9609 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a6a54503c98d131bb71abddbbe06dcaTest https://doi.org/10.1111/1744-7917.12576Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6a6a54503c98d131bb71abddbbe06dca |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17447917 16729609 |
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