Analysis of pir gene expression across the Plasmodium life cycle
| العنوان: | Analysis of pir gene expression across the Plasmodium life cycle |
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| المؤلفون: | Carlos Talavera Lopez, George K. Christophides, Timothy S. Little, Caroline Hosking, Sarah I. Amis, Sarah McLaughlin, Deirdre Cunningham, John W.G. Addy, Christopher Alder, Adam J. Reid, Audrey Vandomme, Jean Langhorne |
| المصدر: | Malaria Journal, Vol 20, Iss 1, Pp 1-14 (2021) Malaria Journal |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Model organisms, Subfamily, Plasmodium berghei, Genes, Protozoan, Interspersed repeat, Immunology, RC955-962, Gene Expression, Infectious Disease, Infectious and parasitic diseases, RC109-216, Biology, Genome, Plasmodium chabaudi, 03 medical and health sciences, 0302 clinical medicine, Arctic medicine. Tropical medicine, Gametocyte, Antigenic variation, Gene, 030304 developmental biology, Genetics, Life Cycle Stages, 0303 health sciences, Research, FOS: Clinical medicine, biology.organism_classification, Infectious Diseases, Multigene Family, Parasitology, 030217 neurology & neurosurgery |
| الوصف: | Background Plasmodium interspersed repeat (pir) is the largest multigene family in the genomes of most Plasmodium species. A variety of functions for the PIR proteins which they encode have been proposed, including antigenic variation, immune evasion, sequestration and rosetting. However, direct evidence for these is lacking. The repetitive nature of the family has made it difficult to determine function experimentally. However, there has been some success in using gene expression studies to suggest roles for some members in virulence and chronic infection. Methods Here pir gene expression was examined across the life cycle of Plasmodium berghei using publicly available RNAseq data-sets, and at high resolution in the intraerythrocytic development cycle using new data from Plasmodium chabaudi. Results Expression of pir genes is greatest in stages of the parasite which invade and reside in red blood cells. The marked exception is that liver merozoites and male gametocytes produce a very large number of pir gene transcripts, notably compared to female gametocytes, which produce relatively few. Within the asexual blood stages different subfamilies peak at different times, suggesting further functional distinctions. Representing a subfamily of its own, the highly conserved ancestral pir gene warrants further investigation due to its potential tractability for functional investigation. It is highly transcribed in multiple life cycle stages and across most studied Plasmodium species and thus is likely to play an important role in parasite biology. Conclusions The identification of distinct expression patterns for different pir genes and subfamilies is likely to provide a basis for the design of future experiments to uncover their function. |
| اللغة: | English |
| تدمد: | 1475-2875 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a62dbacb404bfe3eb3f61389571af2dTest https://doaj.org/article/9efaea334f8249f48589d96afa34ed43Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6a62dbacb404bfe3eb3f61389571af2d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14752875 |
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