Impact of Incretin Hormone Receptors on Insulin-Independent Glucose Disposal in Model Experiments in Mice
| العنوان: | Impact of Incretin Hormone Receptors on Insulin-Independent Glucose Disposal in Model Experiments in Mice |
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| المؤلفون: | Tina Ovlund, Bo Ahrén, Giovanni Pacini |
| المصدر: | Frontiers in Endocrinology, Vol 12 (2021) Frontiers in Endocrinology |
| بيانات النشر: | Frontiers Media SA, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Blood Glucose, 0301 basic medicine, endocrine system, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, glucose disposal, Incretin, 030209 endocrinology & metabolism, Glucagon-Like Peptide-1 Receptor, Diseases of the endocrine glands. Clinical endocrinology, Fluanisone, Receptors, Gastrointestinal Hormone, Mice, 03 medical and health sciences, Endocrinology, 0302 clinical medicine, Internal medicine, Insulin Secretion, insulin-independent, fluafent, medicine, Diazoxide, Animals, Insulin, Receptor, Original Research, Mice, Knockout, GIP, Chemistry, Wild type, RC648-665, diazoxide, Glucose, 030104 developmental biology, Knockout mouse, GLP-1, hormones, hormone substitutes, and hormone antagonists, medicine.drug, Hormone |
| الوصف: | A large contribution to glucose elimination from the circulation is achieved by insulin-independent processes. We have previously shown that the two incretin hormones, glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) increase this process and, therefore, seem to contribute to glucose disposal both through this effect and through the classical incretin effect resulting in enhanced insulin levels. We have now explored in more detail the potential contribution by incretin hormone receptors to insulin-independent processes for glucose elimination. To that end, we have performed intravenous glucose tests (0.35g/kg) in C57BL/6J mice and analyzed glucose elimination rate and glucose effectiveness (i.e., insulin-independent glucose disposal, SG) in wildtype mice and in mice with genetic deletion of GIP receptors or GLP-1 receptors. We performed studies with or without complete blockade of insulin secretion by the drug diazoxide (25 mg/kg). The mice were anesthetized with a novel fentanyl citrate/fluanisone formulation, called Fluafent, together with midazolam. Initially we demonstrated that glucose and insulin data after intravenous and oral glucose were not different using this anesthesia compared to the previously commonly used combination of HypnormR and midazolam. The results show that SG was reduced in GLP-1 receptor knockout mice, whereas there was no difference between GIP receptor knockout mice and wildtype mice, and this was evident both under normal conditions and after complete inhibition of insulin secretion. The study therefore indicates that insulin-independent glucose elimination requires active GLP-1 receptors and thus that the two incretin hormone receptor types show dissociated relevance for this process. |
| تدمد: | 1664-2392 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::695908a3168852282c58e80a5540e17bTest https://doi.org/10.3389/fendo.2021.680153Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....695908a3168852282c58e80a5540e17b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16642392 |
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