Simultaneous quantification of levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin in microvolumes of human plasma using high‐performance liquid chromatography with ultraviolet detection
| العنوان: | Simultaneous quantification of levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin in microvolumes of human plasma using high‐performance liquid chromatography with ultraviolet detection |
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| المؤلفون: | Sihem Benaboud, Ziqing Wang, Gabrielle Lui, Déborah Hirt, Radia Aboura, Jean-Marc Tréluyer, Inès Gana, Yi Zheng |
| المصدر: | Biomedical Chromatography. 33:e4506 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Adolescent, Clinical Biochemistry, 030226 pharmacology & pharmacy, 01 natural sciences, Biochemistry, High-performance liquid chromatography, Pefloxacin, Analytical Chemistry, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Drug Stability, Pharmacokinetics, Limit of Detection, Levofloxacin, Moxifloxacin, Drug Discovery, medicine, Humans, Child, Molecular Biology, Chromatography, High Pressure Liquid, Aged, Aged, 80 and over, Pharmacology, Chromatography, medicine.diagnostic_test, Chemistry, 010401 analytical chemistry, Infant, Newborn, Infant, Reproducibility of Results, General Medicine, Middle Aged, bacterial infections and mycoses, Gatifloxacin, 0104 chemical sciences, Ciprofloxacin, Therapeutic drug monitoring, Child, Preschool, Linear Models, Spectrophotometry, Ultraviolet, Fluoroquinolones, medicine.drug |
| الوصف: | Levofloxacin, pefloxacin, ciprofloxacin and moxifloxacin are four fluoroquinolones used in the treatment of serious bacterial infections. The antibacterial activity of fluoroquinolones is concentration dependent. Therefore, therapeutic drug monitoring in daily clinical practice is warranted to ensure the therapy's efficacy and prevent bacterial resistance. The purpose of the present study was to develop a method using high-pressure liquid chromatography with an ultraviolet detector for simultaneous quantification of these four fluoroquinolones in human plasma. A 50 μL aliquot of plasma was precipitated by 200 μL of methanol using gatifloxacin as internal standard. The chromatographic separation was performed on a Kinetex XB-C18 column using a mobile phase composed of a mixture of orthophosphoric acid 0.4% (v/v), acetonitrile and methanol at a flow rate of 1.2 mL/min. Dual UV wavelength mode was used, with levofloxacin and moxifloxacin monitored at 293 nm, and pefloxacin and ciprofloxacin monitored at 280 nm. The calibration was linear over the ranges of 0.125-25 mg/L for levofloxacin, 0.1-20mg/L for moxifloxacin and 0.05-10 mg/L for both pefloxacin and ciprofloxacin. Inter- and intra-day trueness and precision were |
| تدمد: | 1099-0801 0269-3879 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68e07bb2cb315cec16f6a576bc179b76Test https://doi.org/10.1002/bmc.4506Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....68e07bb2cb315cec16f6a576bc179b76 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10990801 02693879 |
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