Type‐2 diabetes down‐regulates glucose transporter proteins and genes of the human blood leukocytes
| العنوان: | Type‐2 diabetes down‐regulates glucose transporter proteins and genes of the human blood leukocytes |
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| المؤلفون: | Emin Turkay Korgun, S Bilmen-Sarikcioglu, Hasan Altunbaş, Dijle Kipmen-Korgun, Ramazan Demir |
| المصدر: | Scandinavian Journal of Clinical and Laboratory Investigation. 69:350-358 |
| بيانات النشر: | Informa UK Limited, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Clinical Biochemistry, Glucose Transport Proteins, Facilitative, Down-Regulation, Type 2 diabetes, Immune system, Western blot, Diabetes mellitus, Internal medicine, Leukocytes, medicine, Humans, RNA, Messenger, Glucose Transporter Type 1, Glucose Transporter Type 4, Glucose Transporter Type 3, biology, medicine.diagnostic_test, Reverse Transcriptase Polymerase Chain Reaction, Glucose transporter, nutritional and metabolic diseases, General Medicine, Middle Aged, Flow Cytometry, medicine.disease, Endocrinology, Diabetes Mellitus, Type 2, Immunology, biology.protein, Female, GLUT1, hormones, hormone substitutes, and hormone antagonists, GLUT4, GLUT3 |
| الوصف: | White blood cells are essential in mediating immune and inflammatory responses. A prominent feature of these cells during activation of the immune function is increased glucose utilization, and this is dependent on the functioning of specific glucose transporter (GLUT) isoforms. The few data available on leukocyte glucose transporter expression are limited to type-2 diabetes mellitus, and nothing is known about its regulation.Peripheral blood was drawn from 35 healthy controls and 35 diabetic subjects. Expression of GLUT1, GLUT3 and GLUT4 was determined in the leukocytes of healthy individuals and diabetic patients by flow cytometry, Western blot and semi-quantitative RT-PCR.GLUT 3 was decreased in granulocytes, lymphocytes and monocytes from diabetic patients. In monocytes, GLUT3 and GLUT4 were reduced in type-2 diabetic patients. In leukocytes of diabetic patients, GLUT1 and GLUT4, protein and mRNA were unchanged, but GLUT3 protein and mRNA levels were down-regulated compared to those of healthy controls.Elevated glucose concentration affects leukocyte GLUT expression. Decreased expression of GLUT isoforms in leukocytes may be responsible for diminished activation of diabetic leukocytes. These situations possibly contribute to a predisposition to infection and to a decreased immune response in diabetes. |
| تدمد: | 1502-7686 0036-5513 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::686f5a80baf8e0c9026e3e2dbc29732eTest https://doi.org/10.1080/00365510802632163Test |
| رقم الانضمام: | edsair.doi.dedup.....686f5a80baf8e0c9026e3e2dbc29732e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15027686 00365513 |
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