Boosting bacteria differentiation efficiency with multidimensional surface‐enhanced Raman scattering: the example of Bacillus cereus
| العنوان: | Boosting bacteria differentiation efficiency with multidimensional surface‐enhanced Raman scattering: the example of Bacillus cereus |
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| المؤلفون: | Chengye Zhu, Wen Liu, Dongmei Wang, Zhengjun Gong, Meikun Fan |
| المصدر: | Luminescence. 37:1145-1151 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Staphylococcus aureus, Silver, Bacillus cereus, Artificial Intelligence, Chemistry (miscellaneous), Biophysics, Metal Nanoparticles, Spectrum Analysis, Raman |
| الوصف: | Surface-enhanced Raman scattering (SERS) is a powerful tool for constructing biomolecular fingerprints, which play a vital role in differentiation of bacteria. Due to the rather subtle differences in the SERS spectra among different bacteria, artificial intelligence is usually adopted and enormous amounts of spectral data are required to improve the differentiation efficiency. However, in many cases, large volume data acquisition on bacteria is not only technical difficult but labour intensive. It is known that surface modification of SERS nanomaterials can bring additional dimensionality (difference) of the SERS fingerprints. Here in this work, we show that the concept could be used to improve the bacteria differentiation efficiency. Ag NPs were modified with 11-mercaptoundecanoic acid, 11-mercapto-1-undecanol, and 1-dodecanethiol to provide additional dimensionality. The modified NPs then were mixed with cell lysate from different strains of Bacillus cereus (B. cereus). Even by applying a simple PCA process to the resulting SERS spectra data, all the three modified Ag NPs showed superior differentiation results compared with bare Ag NPs, which could only separate Staphylococcus aureus (S. aureus) and B. cereus. It is believed that the multidimensional SERS could find great potential in bacteria differentiation. |
| تدمد: | 1522-7243 1522-7235 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::673babd0fe02d8c17b52627267e3c4ccTest https://doi.org/10.1002/bio.4268Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....673babd0fe02d8c17b52627267e3c4cc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15227243 15227235 |
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