Metabolism-Based Gene Differences in Neurons Expressing Hyperphosphorylated AT8− Positive (AT8+) Tau in Alzheimer’s Disease
| العنوان: | Metabolism-Based Gene Differences in Neurons Expressing Hyperphosphorylated AT8− Positive (AT8+) Tau in Alzheimer’s Disease |
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| المؤلفون: | Angela Everhart, Carol A. Colton, Kirby W Gottschalk, Michael P. Vitek, Audra York |
| المصدر: | ASN NEURO ASN Neuro, Vol 13 (2021) |
| بيانات النشر: | SAGE Publications, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Arginine, Apolipoprotein E4, Neurosciences. Biological psychiatry. Neuropsychiatry, tau Proteins, Biology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Alzheimer Disease, Gene expression, Humans, Phosphorylation, differential gene expression, Gene, Neurons, Original Paper, Methionine, General Neuroscience, Metabolism, radical s-adenosyl domain 1, APOE and sex based changes, Cell biology, Metabolic pathway, 030104 developmental biology, chemistry, brain metabolism, biology.protein, hyperphosphorylated tau, Immunohistochemistry, Female, Neurology (clinical), NeuN, 030217 neurology & neurosurgery, RC321-571 |
| الوصف: | Metabolic adaptations in the brain are critical to the establishment and maintenance of normal cellular functions and to the pathological responses to disease processes. Here, we have focused on specific metabolic pathways that are involved in immune-mediated neuronal processes in brain using isolated neurons derived from human autopsy brain sections of normal individuals and individuals diagnosed as Alzheimer’s disease (AD). Laser capture microscopy was used to select specific cell types in immune-stained thin brain sections followed by NanoString technology to identify and quantify differences in mRNA levels between age-matched control and AD neuronal samples. Comparisons were also made between neurons isolated from AD brain sections expressing pathogenic hyperphosphorylated AT8- positive (AT8+) tau and non-AT8+ AD neurons using double labeling techniques. The mRNA expression data showed unique patterns of metabolic pathway expression between the subtypes of captured neurons that involved membrane based solute transporters, redox factors, and arginine and methionine metabolic pathways. We also identified the expression levels of a novel metabolic gene, Radical-S-Adenosyl Domain1 ( RSAD1) and its corresponding protein, Rsad1, that impact methionine usage and radical based reactions. Immunohistochemistry was used to identify specific protein expression levels and their cellular location in NeuN+ and AT8+ neurons. APOE4 vs APOE3 genotype-specific and sex-specific gene expression differences in these metabolic pathways were also observed when comparing neurons from individuals with AD to age-matched individuals. |
| اللغة: | English |
| تدمد: | 1759-0914 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::666cffab2c00b0dd3a93b5f25eee7578Test http://europepmc.org/articles/PMC8207264Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....666cffab2c00b0dd3a93b5f25eee7578 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17590914 |
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