GDM-Induced Macrosomia Is Reversed by Cav-1 via AMPK-Mediated Fatty Acid Transport and GLUT1-Mediated Glucose Transport in Placenta
| العنوان: | GDM-Induced Macrosomia Is Reversed by Cav-1 via AMPK-Mediated Fatty Acid Transport and GLUT1-Mediated Glucose Transport in Placenta |
|---|---|
| المؤلفون: | Guo Yao, Hui Sang, Yuexia Zhu, Linlin Han, Yeke Tan, Ruirui Yang, Zhanping Shang, Yanyan Lu, Di Wang, Yafang Zhang |
| المصدر: | PLoS ONE PLoS ONE, Vol 12, Iss 1, p e0170490 (2017) |
| بيانات النشر: | Public Library of Science (PLoS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Embryology, endocrine system diseases, Placenta, Maternal Health, Protein Expression, Cell Membranes, Caveolin 1, lcsh:Medicine, AMP-Activated Protein Kinases, Biochemistry, Fetal Macrosomia, Cell Signaling, Glucose Metabolism, AMP-activated protein kinase, Pregnancy, Medicine and Health Sciences, Birth Weight, Phosphorylation, lcsh:Science, Glucose Transporter Type 1, Multidisciplinary, biology, Chemistry, Fatty Acids, Obstetrics and Gynecology, Fatty Acid Transport Proteins, Lipids, medicine.anatomical_structure, Carbohydrate Metabolism, Female, Anatomy, Cellular Structures and Organelles, Research Article, Signal Transduction, Adult, medicine.medical_specialty, Glucose Signaling, Research and Analysis Methods, Young Adult, 03 medical and health sciences, Internal medicine, Gene Expression and Vector Techniques, medicine, Humans, Molecular Biology Techniques, Protein kinase A, Molecular Biology, Molecular Biology Assays and Analysis Techniques, lcsh:R, Reproductive System, Acetyl-CoA carboxylase, Glucose transporter, Biology and Life Sciences, Membrane Proteins, Neonates, nutritional and metabolic diseases, AMPK, Biological Transport, Cell Biology, Diabetes, Gestational, Metabolism, Glucose, 030104 developmental biology, Endocrinology, biology.protein, Women's Health, lcsh:Q, GLUT1, Developmental Biology, Acetyl-CoA Carboxylase |
| الوصف: | Objective To investigate if the role of Cav-1 in GDM-induced macrosomia is through regulating AMPK signaling pathway in placenta. Methods We used diagnostic criteria of gestational diabetes mellitus (GDM) and macrosomia to separate and compare placental protein and mRNA levels from GDM with macrosomia group (GDMM), GDM with normal birth weight group (GDMN) and normal glucose tolerance (NGT) with normal birth weight group (CON). Western blotting was performed to examine differentially expressed proteins of caveolin-1 (Cav-1) and Adenosine monophosphate-activated protein kinase (AMPK) signaling pathway related proteins, including phosphorylated-AMPKα(Thr172), AMPKα, phosphorylated-Acetyl-CoA carboxylase(Ser79) (p-ACC(Ser79)), ACC and glucose transporter 1 (GLUT1) in placenta between the three groups. The mRNA levels of Cav-1, AMPKα, ACC and GLUT1 in placenta were measured by real time-PCR. Results In the GDMM placenta group, both protein and mRNA levels of Cav-1 were down-regulated, while GLUT1 was up-regulated; the phosphorylation and mRNA levels of ACC and AMPKα were decreased, but total ACC protein levels were increased compared to both the GDMN (p |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6659e91b85b300a2b21d59156da7ebadTest https://doi.org/10.1371/journal.pone.0170490Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6659e91b85b300a2b21d59156da7ebad |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
|---|