Stable Oil Bodies Sheltered by a Unique Oleosin in Lily Pollen
| العنوان: | Stable Oil Bodies Sheltered by a Unique Oleosin in Lily Pollen |
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| المؤلفون: | Jason T.C. Tzen, Pei-Luen Jiang, Co-Shing Wang, Guang-Yuh Jauh, Chia-Mei Hsu |
| المصدر: | Plant and Cell Physiology. 48:812-821 |
| بيانات النشر: | Oxford University Press (OUP), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | DNA, Complementary, DNA, Plant, Physiology, Molecular Sequence Data, Plant Science, Biology, medicine.disease_cause, Oil body, Pollen, Botany, medicine, Plant Oils, Protein Isoforms, Amino Acid Sequence, Cloning, Molecular, Peptide sequence, Phylogeny, Pollen maturation, Plant Proteins, Tapetum, Lilium, food and beverages, Cell Biology, General Medicine, Hydrogen-Ion Concentration, biology.organism_classification, Germination, Oleosin |
| الوصف: | Stable oil bodies were purified from mature lily (Lilium longiflorum Thunb.) pollen. The integrity of pollen oil bodies was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Immunodetection revealed that a major protein of 18 kDa was exclusively present in pollen oil bodies and massively accumulated in late stages of pollen maturation. According to mass spectrometric analyses, this oil body protein possessed a tryptic fragment of 13 residues matching that of a theoretical rice oleosin. A complete cDNA fragment encoding this putative oleosin was obtained by PCR cloning with primers derived from its known 13-residue sequence. Sequence analysis as well as immunological non-cross-reactivity suggests that this pollen oleosin represents a distinct class in comparison with oleosins found in seed oil bodies and tapetum. In pollen cells observed by electron microscopy, oil bodies were presumably surrounded by tubular membrane structures, and encapsulated in the vacuoles after germination. It seems that pollen oil bodies are mobilized via a different route from that of glyoxysomal mobilization of seed oil bodies after germination. |
| تدمد: | 1471-9053 0032-0781 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65d55ee6908aa12701b74644a27dd04aTest https://doi.org/10.1093/pcp/pcm051Test |
| رقم الانضمام: | edsair.doi.dedup.....65d55ee6908aa12701b74644a27dd04a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14719053 00320781 |
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