Live attenuated yellow fever 17D infects human DCs and allows for presentation of endogenous and recombinant T cell epitopes
| العنوان: | Live attenuated yellow fever 17D infects human DCs and allows for presentation of endogenous and recombinant T cell epitopes |
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| المؤلفون: | Charles M. Rice, Giovanna Barba-Spaeth, Randy S. Longman, Matthew L. Albert |
| المساهمون: | Laboratory of Virology and Infectious Disease, Rockefeller University [New York]-Center for the Study of Hepatitis C, Immunobiologie des Cellules Dendritiques, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), This work was supported by the Greenberg Medical Research Institute and the Grand Challenges in Global Health. We are grateful to the donors who participated in this study., Vougny, Marie-Christine, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| المصدر: | The Journal of Experimental Medicine Journal of Experimental Medicine Journal of Experimental Medicine, Rockefeller University Press, 2005, 202 (9), pp.1179-84. ⟨10.1084/jem.20051352⟩ Journal of Experimental Medicine, 2005, 202 (9), pp.1179-84. ⟨10.1084/jem.20051352⟩ |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | T-Lymphocytes, Epitopes, T-Lymphocyte, Apoptosis, Epitope, Immunology and Allergy, Neutralizing antibody, Cells, Cultured, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, 0303 health sciences, Antigen Presentation, Vaccines, Synthetic, Attenuated vaccine, MESH: Dendritic Cells, Yellow Fever Vaccine, 3. Good health, medicine.anatomical_structure, MESH: Calcium, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.IMM]Life Sciences [q-bio]/Immunology, Yellow fever virus, medicine.drug, MESH: Cells, Cultured, [SDV.IMM] Life Sciences [q-bio]/Immunology, MESH: Vaccines, Synthetic, T cell, Immunology, Antigen presentation, Yellow fever vaccine, Biology, Vaccines, Attenuated, MESH: Epitopes, T-Lymphocyte, 03 medical and health sciences, Immunity, MESH: Vaccines, Attenuated, medicine, Humans, 030304 developmental biology, MESH: Humans, 030306 microbiology, MESH: Apoptosis, Brief Definitive Report, Dendritic Cells, Virology, MESH: Yellow fever virus, MESH: Yellow Fever Vaccine, MESH: T-Lymphocytes, MESH: Antigen Presentation, biology.protein, Calcium, CD8 |
| الوصف: | International audience; The yellow fever (YF) 17D vaccine is one of the most successful live attenuated vaccines available. A single immunization induces both long-lasting neutralizing antibody and YF-specific T cell responses. Surprisingly, the mechanism for this robust immunity has not been addressed. In light of several recent reports suggesting flavivirus interaction with dendritic cells (DCs), we investigated the mechanism of YF17D interaction with DCs and the importance of this interaction in generating T cell immunity. Our results show that YF17D can infect immature and mature human DCs. Viral entry is Ca(2+) dependent, but it is independent of DC-SIGN as well as multiple integrins expressed on the DC surface. Similar to infection of cell lines, YF infection of immature DCs is cytopathic. Although infection itself does not induce DC maturation in vitro, TNF-alpha-induced maturation protects DCs from YF-induced cytopathogenicity. Furthermore, we show that DCs infected with YF17D or YF17D carrying a recombinant epitope can process and present antigens for CD8(+) T cell stimulation. These findings offer insight into the immunologic mechanisms associated with the highly capable YF17D vaccine that may guide effective vaccine design. |
| وصف الملف: | application/pdf |
| تدمد: | 0022-1007 1540-9538 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65d03e1622480d5d488f4842f61dea9bTest https://pubmed.ncbi.nlm.nih.gov/16260489Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....65d03e1622480d5d488f4842f61dea9b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00221007 15409538 |
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