Enhanced Ghrelin Levels and Hypothalamic Orexigenic AgRP and NPY Neuropeptide Expression in Models of Jejuno-Colonic Short Bowel Syndrome
| العنوان: | Enhanced Ghrelin Levels and Hypothalamic Orexigenic AgRP and NPY Neuropeptide Expression in Models of Jejuno-Colonic Short Bowel Syndrome |
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| المؤلفون: | Laura Gillard, Anne-Charlotte Jarry, Bogdan Stan-Iuga, Maude Le Gall, André Bado, Francisca Joly, Lara Ribeiro-Parenti, Camille Mayeur, Lore Billiauws, Françoise Cluzeaud, Jean-Baptiste Cavin, Jean-Noël Freund, Muriel Thomas, Johanne Le Beyec, Jean-Marc Lacorte |
| المساهمون: | Centre de recherche sur l'Inflammation (CRI (UMR_S_1149 / ERL_8252 / U1149)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Gastroentérologie et d’Assistance nutritive [AP-HP Hôpital Beaujon], Université Paris Diderot - Paris 7 (UPD7)-AP-HP Hôpital Beaujon, Service de Chirurgie Générale et Digestive [Hôpital Bichat - Claude Bernard, Paris], AP-HP - Hôpital Bichat - Claude Bernard [Paris], MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Voies de Signalisation du Développement et du Stress Cellulaire dans les Cancers Digestifs et Urologiques, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Strasbourg (UNISTRA), Université Paris-Sorbonne (UP4), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [APHP], Service de Biochimie Endocrinienne et Oncologique [CHU Pitié-Salpêtrière], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Pitié-Salpêtrière [APHP], Joly, Francisca, Le Beyec, Johanne, Université Paris Diderot - Paris 7 (UPD7)-Hôpital Beaujon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Service de Biochimie Endocrinienne et Oncologie [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), LE GALL, Maude |
| المصدر: | Scientific Reports Scientific Reports, Nature Publishing Group, 2016, Scientific Reports 6, Article number: 28345 (2016), [Epub ahead of print]. ⟨10.1038/srep28345⟩ Scientific Reports (6), 28345. (2016) Scientific Reports, 2016, Scientific Reports 6, Article number: 28345 (2016), [Epub ahead of print]. ⟨10.1038/srep28345⟩ |
| بيانات النشر: | HAL CCSD, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Proglucagon, 0302 clinical medicine, Glucagon-Like Peptide 1, Glucagon-Like Peptide 2, Agouti-Related Protein, Neuropeptide Y, Intestinal Mucosa, contrôle endocrinien, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Multidisciplinary, Anastomosis, Surgical, digestive, oral, and skin physiology, Middle Aged, Short bowel syndrome, Glucagon-like peptide-2, Glucagon-like peptide-1, Jejunum, ghrelin, Female, 030211 gastroenterology & hepatology, Ghrelin, hormones, hormone substitutes, and hormone antagonists, medicine.drug, Adult, Short Bowel Syndrome, medicine.medical_specialty, Colon, Hypothalamus, Neuropeptide, Hyperphagia, Biology, Real-Time Polymerase Chain Reaction, Article, maladie intestinale, 03 medical and health sciences, Orexigenic, Internal medicine, medicine, Animals, Humans, Peptide YY, RNA, Messenger, Rats, Wistar, neuropeptide, Aged, Feeding Behavior, medicine.disease, Rats, Disease Models, Animal, Ki-67 Antigen, 030104 developmental biology, Endocrinology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | Short bowel syndrome (SBS) patients developing hyperphagia have a better outcome. Gastrointestinal endocrine adaptations help to improve intestinal functions and food behaviour. We investigated neuroendocrine adaptations in SBS patients and rat models with jejuno-ileal (IR-JI) or jejuno-colonic (IR-JC) anastomosis with and without parenteral nutrition. Circulating levels of ghrelin, PYY, GLP-1, and GLP-2 were determined in SBS rat models and patients. Levels of mRNA for proglucagon, PYY and for hypothalamic neuropeptides were quantified by qRT-PCR in SBS rat models. Histology and immunostaining for Ki67, GLP-1 and PYY were performed in SBS rats. IR-JC rats, but not IR-JI, exhibited significantly higher crypt depths and number of Ki67-positive cells than sham. Fasting and/or postprandial plasma ghrelin and PYY concentrations were higher, or tend to be higher, in IR-JC rats and SBS-JC patients than in controls. Proglucagon and Pyy mRNA levels were significantly enhanced in IR-JC rats. Levels of mRNA coding hypothalamic orexigenic NPY and AgRP peptides were significantly higher in IR-JC than in sham rats. We demonstrate an increase of plasma ghrelin concentrations, major changes in hypothalamic neuropeptides levels and greater induction of PYY in SBS-JC rats and patients suggesting that jejuno-colonic continuity creates a peculiar environment promoting further gut-brain adaptations. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2045-2322 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::649af728e52b17efaf9bf2f5039f0743Test https://www.hal.inserm.fr/inserm-01346345Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....649af728e52b17efaf9bf2f5039f0743 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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