Carbohydrate improves exercise capacity but does not affect subcellular lipid droplet morphology, AMPK and p53 signalling in human skeletal muscle
| العنوان: | Carbohydrate improves exercise capacity but does not affect subcellular lipid droplet morphology, AMPK and p53 signalling in human skeletal muscle |
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| المؤلفون: | Julien Louis, J. Marc Fell, Sam O. Shepherd, James P. Morton, Emily Jevons, Matthew Cocks, James E. P. Moran, Daniel J. Owens, Daniel G. Ellis, Mark A. Hearris, Juliette A. Strauss |
| المصدر: | The Journal of Physiology. 599:2823-2849 |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_specialty, Physiology, AMP-Activated Protein Kinases, RC1200, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Lipid droplet, Internal medicine, Dietary Carbohydrates, medicine, Humans, Muscle, Skeletal, Meal, Exercise Tolerance, Glycogen, Chemistry, Lactate threshold, Skeletal muscle, AMPK, Lipid Droplets, Carbohydrate, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Mitochondrial biogenesis, Tumor Suppressor Protein p53, 030217 neurology & neurosurgery |
| الوصف: | We examined the effects of carbohydrate (CHO) feeding on lipid droplet (LD) morphology, muscle glycogen utilisation and exercise‐induced skeletal muscle cell signalling. After a 36 h CHO loading protocol and pre‐exercise meal (12 and 2 g kg–1, respectively), eight trained males ingested 0, 45 or 90 g CHO h–1 during 180 min cycling at lactate threshold followed by an exercise capacity test (150% lactate threshold). Muscle biopsies were obtained pre‐ and post‐completion of submaximal exercise. Exercise decreased (P < 0.01) glycogen concentration to comparable levels (∼700 to 250 mmol kg–1 DW), though utilisation was greater in type I (∼40%) versus type II fibres (∼10%) (P < 0.01). LD content decreased in type I (∼50%) and type IIa fibres (∼30%) (P < 0.01), with greater utilisation in type I fibres (P < 0.01). CHO feeding did not affect glycogen or IMTG utilisation in type I or II fibres (all P > 0.05). Exercise decreased LD number within central and peripheral regions of both type I and IIa fibres, though reduced LD size was exclusive to type I fibres. Exercise induced (all P < 0.05) comparable AMPKThr172 (∼4‐fold), p53Ser15 (∼2‐fold) and CaMKIIThr268 phosphorylation (∼2‐fold) with no effects of CHO feeding (all P > 0.05). CHO increased exercise capacity where 90 g h–1 (233 ± 133 s) > 45 g h–1 (156 ± 66 s; P = 0.06) > 0 g h–1 (108 ± 54 s; P = 0.03). In conditions of high pre‐exercise CHO availability, we conclude CHO feeding does not influence exercise‐induced changes in LD morphology, glycogen utilisation or cell signalling pathways with regulatory roles in mitochondrial biogenesis. |
| وصف الملف: | application/pdf |
| تدمد: | 1469-7793 0022-3751 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::646a1f22fc15d4c04153ed7494da6675Test https://doi.org/10.1113/jp281127Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....646a1f22fc15d4c04153ed7494da6675 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14697793 00223751 |
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