Electronegative low density lipoprotein subform is increased in patients with short-duration IDDM and is closely related to glycaemic control
| العنوان: | Electronegative low density lipoprotein subform is increased in patients with short-duration IDDM and is closely related to glycaemic control |
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| المؤلفون: | J. L. Sánchez-Quesada, A. Pérez, A. Caixa`s, J. Ordónmez-Llanos, G. Carreras, A. Payés, F. González-Sastre, A. de Leiva |
| المصدر: | Diabetologia. 39:1469-1476 |
| بيانات النشر: | Springer Science and Business Media LLC, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Adult, Blood Glucose, Male, Electronegative low-density lipoprotein, medicine.medical_specialty, Adolescent, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, chemistry.chemical_compound, Reference Values, Glycation, Internal medicine, Diabetes mellitus, Internal Medicine, medicine, Humans, Insulin, In patient, Vascular disease, Middle Aged, medicine.disease, Lipoproteins, LDL, Diabetes Mellitus, Type 1, Fructosamine, Endocrinology, chemistry, Chromatography, Gel, Female, lipids (amino acids, peptides, and proteins), Density gradient ultracentrifugation, Oxidation-Reduction |
| الوصف: | We evaluated the effect of improving glycaemic control with intensive insulin therapy on LDL susceptibility to oxidation, electronegative LDL proportion, and LDL subfraction phenotype in a group of 25 patients with short-duration insulin-dependent diabetes mellitus (IDDM); 25 matched healthy control subjects were also studied. LDL susceptibility to oxidation was measured by continuous monitoring of conjugated diene formation. Electronegative LDL was isolated by anion exchange chromatography, and quantified as percentage of total LDL. Six LDL subfractions were isolated by density gradient ultracentrifugation and phenotype A or B classified as the quotient (LDL1-LDL3)/(LDL4-LDL6). Compared to the control group, IDDM subjects with poor glycaemic control showed higher electronegative LDL (19.03 ± 10.09 vs 9.59 ± 2.98 %, p < 0.001), similar LDL subfraction phenotype and lower susceptibility to oxidation (lag phase 45.6 ± 8.8 vs 41.2 ± 4.7 min, p < 0.05). After three months of intensive insulin therapy, HbA1 c decreased from 10.88 ± 2.43 to 5.69 ± 1.54 % (p < 0.001), and electronegative LDL to 13.84 ± 5.15 % (p < 0.05). No changes in LDL susceptibility to oxidation or LDL subfraction phenotype were observed. Electronegative LDL appeared significantly correlated to HbA1 c and fructosamine (p < 0.01 and p < 0.001) only in poorly controlled IDDM patients. These findings suggest that high electronegative LDL in IDDM subjects is related to the degree of glycaemic control, and could therefore be due to LDL glycation rather than to LDL oxidation or changes in LDL subfraction phenotype. [Diabetologia (1996) 39: 1469–1476] |
| تدمد: | 1432-0428 0012-186X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::643eb2a122ef78839ab984d76b631df6Test https://doi.org/10.1007/s001250050600Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....643eb2a122ef78839ab984d76b631df6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320428 0012186X |
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