Introduction of an AmpR-L2 intergenic segment attenuates the induced β-lactamase activity of Stenotrophomonas maltophilia
| العنوان: | Introduction of an AmpR-L2 intergenic segment attenuates the induced β-lactamase activity of Stenotrophomonas maltophilia |
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| المؤلفون: | Tung-Ching Chung, Kai Hung Chiang, Yi-Chih Chang, Tsuey Ching Yang, Yi-Wei Huang |
| المصدر: | European Journal of Clinical Microbiology & Infectious Diseases. 29:887-890 |
| بيانات النشر: | Springer Science and Business Media LLC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | DNA, Bacterial, Microbiology (medical), Stenotrophomonas maltophilia, Molecular Sequence Data, Attenuator (genetics), Genetic analysis, beta-Lactamases, Microbiology, Intergenic region, Bacterial Proteins, Enterobacter cloacae, Cluster Analysis, Gene, Phylogeny, Polymorphism, Genetic, biology, Sequence Analysis, DNA, General Medicine, biology.organism_classification, Mutagenesis, Insertional, Infectious Diseases, Pseudomonadales, DNA, Intergenic, Pseudomonadaceae |
| الوصف: | Expression of the L1 and L2 beta-lactamase genes is generally regulated by a LysR type regulator of the AmpR in Stenotrophomonas maltophilia. The ampR gene is located immediately upstream of L2 and is transcribed divergently, forming an ampR-L2 module. The ampR-L2 modules of 16 S. maltophilia isolates were analyzed, revealing that the ampR-L2 intergenic (IG) regions show a significant genetic diversity, whereas AmpR proteins are highly conserved. The induction potential of the different AmpR toward the different ampR-L2 IG regions was evaluated by introducing the various IG-xylE transcriptional fusion constructs into a wild S. maltophilia strain. The induction levels achieved in the various AmpR-IG pairs display quantitative differences; meanwhile, the host beta-lactamase activity, in some cases, is attenuated by the introduced IG segment. Similar beta-lactamase attenuation phenomenon was observed in Enterobacter cloacae with an ampR-L2 IG segment of S. maltophilia. A concept of oligonucleotides attenuator for the development of an antimicrobial agent is proposed. |
| تدمد: | 1435-4373 0934-9723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::631012ec2efc978ed34320b37be46a67Test https://doi.org/10.1007/s10096-010-0924-0Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....631012ec2efc978ed34320b37be46a67 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14354373 09349723 |
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