Decidual Natural Killer Cell Interactions with Trophoblasts Are Impaired in Pregnancies at Increased Risk of Preeclampsia
| العنوان: | Decidual Natural Killer Cell Interactions with Trophoblasts Are Impaired in Pregnancies at Increased Risk of Preeclampsia |
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| المؤلفون: | Alison E. Wallace, Judith E. Cartwright, Guy Whitley, Amanda J Host |
| المصدر: | The American Journal of Pathology. 183:1853-1861 |
| بيانات النشر: | Elsevier BV, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Adult, medicine.medical_specialty, MAP Kinase Signaling System, Cell, Pregnancy Proteins, Biology, Pathology and Forensic Medicine, Natural killer cell, Preeclampsia, Phosphatidylinositol 3-Kinases, 03 medical and health sciences, 0302 clinical medicine, Pre-Eclampsia, Pregnancy, Risk Factors, Internal medicine, Decidua, medicine, Humans, reproductive and urinary physiology, 030304 developmental biology, Mitogen-Activated Protein Kinase 1, 0303 health sciences, Fetus, Mitogen-Activated Protein Kinase 3, 030219 obstetrics & reproductive medicine, Chemotaxis, Placentation, Trophoblast, Regular Article, medicine.disease, Trophoblasts, Killer Cells, Natural, medicine.anatomical_structure, Endocrinology, embryonic structures, Female, Proto-Oncogene Proteins c-akt |
| الوصف: | Transformation of the uterine spiral arteries (SAs) during pregnancy is critical to support the developing fetus, and is impaired in some pregnancy disorders, including preeclampsia. Decidual natural killer (dNK) cells play a role in SA remodeling, although their interactions with fetal trophoblast remain unclear. A uterine artery Doppler resistance index (RI) in the first trimester of pregnancy can be used as a proxy measure of the extent of SA remodeling; we have used this technique to characterize dNK cells from pregnancies with normal (normal RI) and impaired (high RI) SA remodeling, which display least and highest risk of developing preeclampsia, respectively. We examined the impact of dNK cell secreted factors on trophoblast motility, chemoattraction, and signaling pathways to determine the contribution of dNK cells to SA transformation. We demonstrated that the chemoattraction of the trophoblast by dNK cells is impaired in pregnancies with high RI, as is the ability to induce trophoblast outgrowth from placental villous explants. These processes are dependent on activation of the extracellular signal–regulated kinase 1/2 and phosphatidylinositol 3-kinase–Akt signaling pathways, which were altered in trophoblasts incubated with secreted factors from dNK cells from high RI pregnancies. Therefore, by characterizing pregnancies using uterine artery Doppler RI before dNK cell isolation, we have identified that impaired dNK-trophoblast interactions may lead to poor placentation. These findings have implications for pregnancy pathological conditions, such as preeclampsia. |
| وصف الملف: | application/pdf |
| تدمد: | 0002-9440 1525-2191 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62864fb1b5de9380d6a5eec919e19b38Test https://doi.org/10.1016/j.ajpath.2013.08.023Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....62864fb1b5de9380d6a5eec919e19b38 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00029440 15252191 |
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