Rat testicular myoid cells respond to endothelin: characterization of binding and signal transduction pathway
| العنوان: | Rat testicular myoid cells respond to endothelin: characterization of binding and signal transduction pathway |
|---|---|
| المؤلفون: | Rossella Paniccia, Anna Teti, Fioretta Palombi, Mario Stefanini, Antonella Tripiciano, Antonio Filippini, Elio Ziparo |
| المصدر: | Scopus-Elsevier |
| بيانات النشر: | The Endocrine Society, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, Inositol Phosphates, Biology, Paracrine signalling, chemistry.chemical_compound, Endocrinology, Internal medicine, Testis, medicine, Animals, Inositol, Phosphatidylinositol, Autocrine signalling, Cells, Cultured, Binding Sites, Endothelins, Osmolar Concentration, Intracellular Membranes, Sertoli cell, Endothelin 1, Rats, Seminiferous tubule, medicine.anatomical_structure, chemistry, Calcium, Intracellular, Signal Transduction |
| الوصف: | The presence of endothelin (ET), a vasoconstrictor peptide, in the testis suggests that it may regulate nonvascular target cells. We investigated binding ability, regulation of inositol phosphate metabolism, changes in cytosolic free Ca2+ concentrations ([Ca2+]i), and induction of morphological changes by ET-1 in rat primary testicular myoid cell cultures. ET-1 specifically bound to highly purified rat testicular myoid cells in a time- and temperature-dependent manner. Scatchard analysis of the binding studies indicated the presence of a single class of high affinity binding sites, with an apparent Kd of 3 x 10(-10) M and a maximal binding capacity of 10(5) sites/cell. ET-1 induced both rapid production of inositol triphosphate and mobilization of [Ca2+]i in a concentration-dependent fashion. By contrast, inositol lipid metabolism was slightly affected by ET-1 in the total peritubular cell population. Purified Sertoli cells failed to show either ET-1 binding or ET-1-induced phosphatidylinositol hydrolysis. Mobilization of [Ca2+]i mostly depended upon the release of Ca2+ from thapsigargin-sensitive intracellular Ca2+, whereas it was not affected by abolishment of the Ca2+ gradient through the plasma membrane or by inhibition of L-type voltage-sensitive Ca(2+)-channels by nifedipine. These findings together with the fact that Sertoli cells are unable to respond to and bind ET-1 indicate that ET is a specific agonist of myoid cells in the seminiferous tubule and suggest a role for ET-1 in the autocrine/paracrine regulation of testicular function. |
| تدمد: | 1945-7170 0013-7227 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61c2986e4b9132c038c8cc87a202e1feTest https://doi.org/10.1210/endo.133.4.8404621Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....61c2986e4b9132c038c8cc87a202e1fe |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457170 00137227 |
|---|