The superficial layer of human articular cartilage is more susceptible to interleukin-1–induced damage than the deeper layers
| العنوان: | The superficial layer of human articular cartilage is more susceptible to interleukin-1–induced damage than the deeper layers |
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| المؤلفون: | M. B. Aydelotte, E. J-M. A. Thonar, K. E. Kuettner, Johannes Flechtenmacher, L. Michal, H. J. Häuselmann, M. Shinmei |
| المصدر: | Arthritis & Rheumatism. 39:478-488 |
| بيانات النشر: | Wiley, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Adult, Cartilage, Articular, Adolescent, medicine.drug_class, Sialoglycoproteins, Immunology, Matrix metalloproteinase, Chondrocyte, Rheumatology, medicine, Humans, Immunology and Allergy, Pharmacology (medical), Catabolism, Chemistry, Cartilage, Receptors, Interleukin-1, Interleukin, Biological activity, Anatomy, Middle Aged, Receptor antagonist, Recombinant Proteins, Cell biology, Interleukin 1 Receptor Antagonist Protein, medicine.anatomical_structure, Cell culture, Proteoglycans, Interleukin-1 |
| الوصف: | Objective. To compare the responses of chondrocytes from superficial and deep layers of normal human articular cartilage to interleukin-1 (IL-1) and IL-1 receptor antagonist protein (IRAP), and to evaluate the binding sites for IL-1 on these cells. Methods. Cartilage and chondrocytes from superficial and deeper layers of human femoral condyles were cultured with and without IL-1 in the presence and absence of IRAP. The effect of these agents on 35S-proteoglycan synthesis and catabolism and production of stromelysin and tissue inhibitor of metalloproteinases 1 (TIMP-1) were measured by biochemical and immunologic assays. Receptor binding was evaluated using 125I-labeled IL-1. Results. IL-1 induced more severe inhibition of proteoglycan synthesis and a lower ratio of secreted TIMP-1:stromelysin in chondrocytes from superficial cartilage than those from deeper cartilage. IRAP blocked responses to IL-1 more effectively in chondrocytes from deep cartilage than those from superficial cartilage. Chondrocytes from the articular surface showed approximately twice the number of high-affinity binding sites for IL-1 as did cells from deep cartilage. Conclusion. Chondrocytes from the surface of articular cartilage show a greater vulnerability to the harmful effects of IL-1 and are less responsive to the potential therapeutic effects of IRAP than cells in the deeper layers of the tissue. |
| تدمد: | 1529-0131 0004-3591 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::615f8af66fb4322d3d6db9bbd2ff8677Test https://doi.org/10.1002/art.1780390316Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....615f8af66fb4322d3d6db9bbd2ff8677 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15290131 00043591 |
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