Clinicomolecular Identification of Conserved and Individualized Features of Granulomatous Uveitis
| العنوان: | Clinicomolecular Identification of Conserved and Individualized Features of Granulomatous Uveitis |
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| المؤلفون: | Lacey Feigl-Lenzen, Maxim N. Artyomov, Grace L. Paley, Cynthia L. Montana, Lynn M. Hassman, Wayne M. Yokoyama, Philip A. Ruzycki, Karen Hong, Luke E. Springer, Nicole Linskey, Hayley James, Jennifer M. Enright, Jennifer Laurent, Ekaterina Esaulova, Michael A. Paley |
| المصدر: | Ophthalmol Sci Ophthalmology Science, Vol 1, Iss 1, Pp 100010-(2021) |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | B cells, biology, Innate lymphoid cell, B-cell receptor, T cells, clonal expansion, General Medicine, Human leukocyte antigen, RE1-994, medicine.disease, Major histocompatibility complex, Article, Uveitis, Ophthalmology, Immune system, Immunoglobulin class switching, single cell RNA sequencing, Immunology, medicine, biology.protein, Cytotoxic T cell |
| الوصف: | Purpose To identify molecular features that distinguish individuals with shared clinical features of granulomatous uveitis. Design Cross-sectional observational study. Participants Four eyes from patients with active granulomatous uveitis. Methods We performed single-cell RNA sequencing with antigen-receptor sequence analysis to obtain an unbiased gene expression survey of ocular immune cells and to identify clonally expanded lymphocytes. Main Outcomes Measures For each inflamed eye, we measured the proportion of distinct immune cell types, the amount of B- or T-cell clonal expansion, and the transcriptional profile of T and B cells. Results Each individual showed robust clonal expansion arising from a single T- or B-cell lineage, suggesting distinct, antigen-driven pathogenic processes in each patient. This variability in clonal expansion was mirrored by individual variability in CD4 T-cell populations, whereas ocular CD8 T cells and B cells were more similar transcriptionally among patients. Finally, ocular B cells displayed evidence of class switching and plasmablast differentiation within the ocular microenvironment, providing additional support for antigen-driven immune responses in granulomatous uveitis. Conclusions Collectively, our study identified both conserved and individualized features of granulomatous uveitis, illuminating parallel pathophysiologic mechanisms and suggesting that future personalized therapeutic approaches may be warranted. |
| تدمد: | 2666-9145 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60553a85d13c8fb89ec71448ed8ce859Test https://pubmed.ncbi.nlm.nih.gov/35937550Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....60553a85d13c8fb89ec71448ed8ce859 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26669145 |
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