DNA methylation alterations in grade II- and anaplastic pleomorphic xanthoastrocytoma
| العنوان: | DNA methylation alterations in grade II- and anaplastic pleomorphic xanthoastrocytoma |
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| المؤلفون: | Matthias Kirsch, Miguel Vizoso, Veit Rohde, F. Javier Carmona, Alonso Barrantes, Santiago Ropero, Ramon Martinez, Manel Esteller, Werner Paulus, Antonio Gomez, Gabriele Schackert |
| المساهمون: | Universitat de Barcelona |
| المصدر: | Recercat. Dipósit de la Recerca de Catalunya instname Dipòsit Digital de la UB Universidad de Barcelona BMC Cancer |
| بيانات النشر: | Springer Science and Business Media LLC, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Male, Cancer Research, Pathology, ADN, medicine.disease_cause, Epigenesis, Genetic, Malignant transformation, 0302 clinical medicine, Basic Helix-Loop-Helix Transcription Factors, Promoter Regions, Genetic, Càncer, Cancer, Pleomorphic xanthoastrocytoma, DNA methylation, RNA-Binding Proteins, Astrocytoma, DNA, Neoplasm, Methylation, LIM Domain Proteins, Middle Aged, Pleomorphic xanthoastrocyma, Gene Expression Regulation, Neoplastic, Oncology, 030220 oncology & carcinogenesis, Proto-Oncogene Proteins c-hck, Epigenetics, Female, Metilació, Research Article, Adult, medicine.medical_specialty, Adolescent, Biology, Tetraspanin 28, Young Adult, 03 medical and health sciences, Genetics, medicine, Humans, Gene, Aged, Tumors, Homeodomain Proteins, Sequence Analysis, DNA, DNA, medicine.disease, Cytoskeletal Proteins, Cancer research, Glioblastoma, Carcinogenesis, Genètica, 030217 neurology & neurosurgery |
| الوصف: | Background Pleomorphic xanthoastrocytoma (PXA) is a rare WHO grade II tumor accounting for less than 1% of all astrocytomas. Malignant transformation into PXA with anaplastic features, is unusual and correlates with poorer outcome of the patients. Methods Using a DNA methylation custom array, we have quantified the DNA methylation level on the promoter sequence of 807 cancer-related genes of WHO grade II (n = 11) and III PXA (n = 2) and compared to normal brain tissue (n = 10) and glioblastoma (n = 87) samples. DNA methylation levels were further confirmed on independent samples by pyrosequencing of the promoter sequences. Results Increasing DNA promoter hypermethylation events were observed in anaplastic PXA as compared with grade II samples. We further validated differential hypermethylation of CD81, HCK, HOXA5, ASCL2 and TES on anaplastic PXA and grade II tumors. Moreover, these epigenetic alterations overlap those described in glioblastoma patients, suggesting common mechanisms of tumorigenesis. Conclusions Even taking into consideration the small size of our patient populations, our data strongly suggest that epigenome-wide profiling of PXA is a valuable tool to identify methylated genes, which may play a role in the malignant progression of PXA. These methylation alterations may provide useful biomarkers for decision-making in those patients with low-grade PXA displaying a high risk of malignant transformation. peerReviewed |
| وصف الملف: | application/pdf |
| تدمد: | 1471-2407 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6033c2cb3461821e1b827b97996a1056Test https://doi.org/10.1186/1471-2407-14-213Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6033c2cb3461821e1b827b97996a1056 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712407 |
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