Long non-coding RNA BCYRN1 promotes glycolysis and tumor progression by regulating the miR-149/PKM2 axis in non-small-cell lung cancer
| العنوان: | Long non-coding RNA BCYRN1 promotes glycolysis and tumor progression by regulating the miR-149/PKM2 axis in non-small-cell lung cancer |
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| المؤلفون: | Jiangyang Zhao, Ning Lang, Feng Shi, Chunyang Wang, Hongyan Cao, Tong Wu |
| المصدر: | Molecular Medicine Reports |
| بيانات النشر: | D.A. Spandidos, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Adult, Male, Cancer Research, Thyroid Hormones, Lung Neoplasms, Cell, PKM2, Biochemistry, brain cytoplasmic RNA 1, 03 medical and health sciences, 0302 clinical medicine, Genes, Reporter, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, microRNA, Genetics, medicine, Humans, Molecular Biology, 3' Untranslated Regions, Matrigel Invasion Assay, Cell growth, Chemistry, Membrane Proteins, Articles, Cell cycle, glycolysis, Middle Aged, respiratory tract diseases, Gene Expression Regulation, Neoplastic, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, Oncology, non-small-cell lung cancer, Anaerobic glycolysis, 030220 oncology & carcinogenesis, Cancer cell, Cancer research, Disease Progression, Molecular Medicine, pyruvate kinase M1/2, Female, RNA Interference, RNA, Long Noncoding, microRNA-149, Carrier Proteins |
| الوصف: | Cancer cells use aerobic glycolysis to sustain their proliferation. Long non‑coding RNA brain cytoplasmic RNA 1 (BCYRN1) has been reported to act as an oncogene in non‑small‑cell lung cancer (NSCLC). The present study investigated the role of BCYRN1 in NSCLC glycolysis. BCYRN1 expression was detected in NSCLC cells and tissues using reverse transcription‑quantitative PCR. The effect of BCYRN1 on aerobic glycolysis was examined by measuring NSCLC cell glucose catabolism and lactate synthesis. The relationships between BCYRN1 and microRNA (miR)‑149, and between miR‑149 and pyruvate kinase M1/2 (PKM2) were measured using a dual‑luciferase reporter assay. Cell proliferation and invasion were analyzed by the Cell Counting kit‑8 assay and the Matrigel invasion assay, respectively. High BCYRN1 expression was observed in NSCLC tissues and cells compared with the corresponding controls. BCYRN1 induced glycolysis and upregulated the expression levels of PKM2 in NSCLC cells. In addition, BCYRN1 regulated miR‑149 expression levels, and miR‑149 inhibitor rescued the effects of si‑BCYRN1 on glucose consumption and lactate production. miR‑149 knockdown significantly enhanced the expression of PKM2. Furthermore, PKM2 inhibition significantly reversed the effects of miR‑149 inhibitor on glucose catabolism and lactate synthesis. Furthermore, PKM2 was involved in NSCLC cell proliferation and invasion, and BCYRN1 knockdown and miR‑149 overexpression inhibited both processes. The present study suggested that BCYRN1 was involved in cell glycolysis, proliferation and invasion during NSCLC via regulating miR‑149 and PKM2. |
| اللغة: | English |
| تدمد: | 1791-3004 1791-2997 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e809e129f6276bc28cdab313c2ea9d3Test http://europepmc.org/articles/PMC7003037Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5e809e129f6276bc28cdab313c2ea9d3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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