The usefulness of a novel fully automated PCR-based Idylla test for detection of the BRAF V600E mutation in thyroid tissue: comparison with PNA-clamping PCR, real-time PCR and pyrosequencing
| العنوان: | The usefulness of a novel fully automated PCR-based Idylla test for detection of the BRAF V600E mutation in thyroid tissue: comparison with PNA-clamping PCR, real-time PCR and pyrosequencing |
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| المؤلفون: | Gang Min Hur, Su Yel Lee, Min-Kyung Yeo, Minkyu Jung, Jin-Man Kim, Yong-Moon Lee |
| المصدر: | Journal of Clinical Pathology. 70:260-265 |
| بيانات النشر: | BMJ, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Proto-Oncogene Proteins B-raf, 0301 basic medicine, Genotyping Techniques, endocrine system diseases, DNA Mutational Analysis, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Pathology and Forensic Medicine, Thyroid carcinoma, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Thyroid Neoplasms, neoplasms, business.industry, Molecular pathology, Carcinoma, Thyroid, Reproducibility of Results, General Medicine, Molecular biology, Carcinoma, Papillary, digestive system diseases, BRAF V600E, 030104 developmental biology, medicine.anatomical_structure, Real-time polymerase chain reaction, Fully automated, Thyroid Cancer, Papillary, 030220 oncology & carcinogenesis, Mutation (genetic algorithm), Pyrosequencing, business |
| الوصف: | Introduction The BRAF V600E mutation is the most common genetic event in papillary thyroid carcinoma (PTC). The BRAF V600E mutational status has a significant diagnostic and prognostic role in PTC since it can be detected in 32%–87% of PTC by various molecular methods. Aim(s) A novel, fully automated real-time PCR-based Idylla test is assessed to detect the BRAF mutation in formalin-fixed paraffin-embedded (FFPE) thyroid samples. Methods 99 PTC and 11 nodular hyperplasia FFPE thyroid tissues are evaluated for the BRAF V600E mutation by the Idylla tests and compared with peptide nucleic acid-clamping PCR, real-time PCR and pyrosequencing. Results The sensitivity and specificity of the Idylla test to detect BRAF V600E are 98.8% and 100%, which is superior to real-time PCR and pyrosequencing. The concordance between Idylla and true positive is highest at 0.974. Conclusions This study validates that the Idylla test is a sensitive and specific method to detect BRAF V600E in FFPE thyroid tissues. A simple, quick and easy to handle Idylla test is a useful and reliable molecular technique to evaluate BRAF mutations. |
| تدمد: | 1472-4146 0021-9746 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e6a76ec411e1c094c8d638b0c6ee835Test https://doi.org/10.1136/jclinpath-2016-204025Test |
| رقم الانضمام: | edsair.doi.dedup.....5e6a76ec411e1c094c8d638b0c6ee835 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14724146 00219746 |
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