Biofilm Production and Antibiofilm Activity of Echinocandins and Liposomal Amphotericin B in Echinocandin-Resistant Yeast Species
| العنوان: | Biofilm Production and Antibiofilm Activity of Echinocandins and Liposomal Amphotericin B in Echinocandin-Resistant Yeast Species |
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| المؤلفون: | Laura Judith Marcos-Zambrano, Pilar Escribano, Oscar Zaragoza, Emilio Bouza, Jesús Guinea, Marta Gomez-Perosanz |
| المصدر: | Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid Consejería de Sanidad de la Comunidad de Madrid |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0301 basic medicine, Antifungal Agents, Echinocandin, 030106 microbiology, Microbial Sensitivity Tests, Anidulafungin, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Echinocandins, Lipopeptides, Caspofungin, Drug Resistance, Fungal, Amphotericin B, medicine, polycyclic compounds, Humans, Pharmacology (medical), Fungemia, Candida, Pharmacology, Micafungin, Biofilm, Fungal genetics, Candidiasis, biochemical phenomena, metabolism, and nutrition, medicine.disease, bacterial infections and mycoses, Infectious Diseases, chemistry, Glucosyltransferases, Susceptibility, Biofilms, medicine.drug |
| الوصف: | The echinocandins and liposomal amphotericin B are active against biofilm produced by echinocandin-susceptible Candida strains. However, few data have been reported on the production of biofilm by echinocandin-resistant isolates and their antifungal susceptibility. We studied the production of biofilm by fks mutant Candida strains and intrinsically echinocandin-resistant non- Candida isolates and the susceptibility of both entities to liposomal amphotericin B and echinocandins. We analyzed the production of biofilm by isolates from patients with fungemia ( fks mutant Candida , n = 5; intrinsically echinocandin-resistant non- Candida , n = 12; and Candida wild type, n = 10). Biofilm formation was measured to classify strains according to biomass (crystal violet assay) and metabolic activity (XTT reduction assay). Preformed biofilms were tested against liposomal amphotericin B, caspofungin, micafungin, and anidulafungin. The sessile MIC was defined as the antifungal concentration yielding a 50% or 80% reduction in the metabolic activity of the biofilm compared to that of the growth control (SMIC 50 and SMIC 80 , respectively). fks mutant Candida isolates formed biofilms in a fashion similar to that of Candida wild-type strains. The echinocandins had the highest activity against biofilms formed by wild-type Candida isolates, followed by fks mutant Candida isolates and non- Candida isolates. Liposomal amphotericin B had the highest activity against fks mutant Candida biofilms. The formation of biofilm by echinocandin-resistant strains was similar to that of wild-type strains, although resistance to echinocandins remained high. |
| وصف الملف: | application/pdf |
| تدمد: | 1098-6596 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5dd08fcae0bed14fbbf26ef123fbe736Test https://pubmed.ncbi.nlm.nih.gov/27021323Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5dd08fcae0bed14fbbf26ef123fbe736 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10986596 |
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