Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease
| العنوان: | Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease |
|---|---|
| المؤلفون: | Feng Zhang, Silvana Konermann, James E. Dahlman, Omar O. Abudayyeh, Jonathan S. Gootenberg, Julia Joung |
| المساهمون: | Harvard University--MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences, McGovern Institute for Brain Research at MIT, Abudayyeh, Omar Osama, Joung, Julia, Gootenberg, Jonathan S., Zhang, Feng, Konermann, Silvana |
| المصدر: | PMC Nature biotechnology |
| بيانات النشر: | Nature Publishing Group, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Biomedical Engineering, Bioengineering, Applied Microbiology and Biotechnology, Article, 03 medical and health sciences, Gene Knockout Techniques, 0302 clinical medicine, Bacterial Proteins, CRISPR-Associated Protein 9, Gene expression, CRISPR, Humans, Gene, Gene knockout, 030304 developmental biology, 0303 health sciences, Nuclease, biology, Cas9, HEK 293 cells, Endonucleases, Molecular biology, 3. Good health, HEK293 Cells, biology.protein, Molecular Medicine, 030217 neurology & neurosurgery, Biotechnology, RNA, Guide, Kinetoplastida |
| الوصف: | We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loops, can activate gene expression using an active Streptococcus pyogenes Cas9 nuclease, without inducing double-stranded breaks. We use these 'dead RNAs' to perform orthogonal gene knockout and transcriptional activation in human cells. McGovern Institute for Brain Research at MIT (Friends of McGovern Institute Fellowship) United States. Dept. of Energy (Computational Science Graduate Fellowship) National Institute of Mental Health (U.S.) (1DP1-MH100706) Poitras Foundation Vallee Foundation Simons Foundation Paul G. Allen Foundation New York Stem Cell Foundation |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ca80b9c6e2ed270412348b27cfa0bf7Test https://orcid.org/0000-0001-6656-5002Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5ca80b9c6e2ed270412348b27cfa0bf7 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |