miR-21 Is Linked to Glioma Angiogenesis
| العنوان: | miR-21 Is Linked to Glioma Angiogenesis |
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| المؤلفون: | Charlotte Aaberg-Jessen, Boye Schnack Nielsen, Simon Kjær Hermansen, Bjarne Winther Kristensen |
| المصدر: | Hermansen, S K, Nielsen, B S, Aaberg-Jessen, C & Kristensen, B W 2016, ' miR-21 Is Linked to Glioma Angiogenesis : A Co-Localization Study ', Journal of Histochemistry and Cytochemistry, vol. 64, no. 2, pp. 138-148 . https://doi.org/10.1369/0022155415623515Test |
| بيانات النشر: | SAGE Publications, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Male, Vascular Endothelial Growth Factor A, 0301 basic medicine, Pathology, medicine.medical_specialty, Histology, Angiogenesis, Astrocytoma, Stem cell marker, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Cancer stem cell, Glioma, Image Processing, Computer-Assisted, Journal Article, medicine, Humans, PTEN, Tensin, In Situ Hybridization, Aged, Neovascularization, Pathologic, Staining and Labeling, biology, Brain Neoplasms, Research Support, Non-U.S. Gov't, PTEN Phosphohydrolase, Brain, Articles, Middle Aged, Hypoxia-Inducible Factor 1, alpha Subunit, medicine.disease, Vascular endothelial growth factor, MicroRNAs, Vascular endothelial growth factor A, 030104 developmental biology, chemistry, 030220 oncology & carcinogenesis, Neoplastic Stem Cells, biology.protein, Cancer research, Female, Anatomy |
| الوصف: | MicroRNA-21 (miR-21) is the most consistently over-expressed microRNA (miRNA) in malignant gliomas. We have previously reported that miR-21 is upregulated in glioma vessels and subsets of glioma cells. To better understand the role of miR-21 in glioma angiogenesis and to characterize miR-21-positive tumor cells, we systematically stained consecutive serial sections from ten astrocytomas for miR-21, hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), phosphatase and tensin homolog (PTEN), octamer-binding transcription factor 4 (Oct4), sex-determining region Y box 2 (Sox2) and CD133. We developed an image analysis-based co-localization approach allowing global alignment and quantitation of the individual markers, and measured the miR-21 in situ hybridization signal against the immunohistochemical staining of the six different markers. miR-21 significantly co-localized with the hypoxia- and angiogenesis-associated markers HIF-1α ( p=0.0020) and VEGF ( p=0.0096), whereas the putative miR-21 target, PTEN, was expressed independently of miR-21. Expression of stem cell markers Oct4, Sox2 and CD133 was not associated with miR-21. In six glioblastoma cultures, miR-21 did not correlate with the six markers. These findings suggest that miR-21 is linked to glioma angiogenesis, that miR-21 is unlikely to regulate PTEN, and that miR-21-positive tumor cells do not possess stem cell characteristics. |
| تدمد: | 1551-5044 0022-1554 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b899b45ac85d2138be5038a41f6405cTest https://doi.org/10.1369/0022155415623515Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5b899b45ac85d2138be5038a41f6405c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15515044 00221554 |
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