Role of Pituitary Tumour Transforming Gene 1 in Medullary Thyroid Carcinoma
| العنوان: | Role of Pituitary Tumour Transforming Gene 1 in Medullary Thyroid Carcinoma |
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| المؤلفون: | Maria Chiara Zatelli, Federico Tagliati, Vincenzo Amodio, Mattia Buratto, Mariarosa Pelizzo, Giancarlo Pansini, Marta Bondanelli, Maria Rosaria Ambrosio, Ettore C. degli Uberti |
| المصدر: | Analytical Cellular Pathology, Vol 33, Iss 5-6, Pp 207-216 (2010) Analytical Cellular Pathology (Amsterdam) |
| بيانات النشر: | Hindawi Limited, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Adult, Male, Cancer Research, endocrine system, Adolescent, Pituitary tumour transforming gene 1, Blotting, Western, Pathology and Forensic Medicine, medullary thyroid carcinoma, Biomarkers, Tumor, Humans, Gene Silencing, Thyroid Neoplasms, Child, RC254-282, Aged, Cell Proliferation, Neoplasm Staging, Hyperplasia, QH573-671, Reverse Transcriptase Polymerase Chain Reaction, TT cells, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Cell Biology, General Medicine, Middle Aged, Blotting, Northern, Carcinoma, Neuroendocrine, Neoplasm Proteins, Securin, Microscopy, Fluorescence, siRNA, Lymphatic Metastasis, Molecular Medicine, Female, Other, Cytology |
| الوصف: | Background: Pituitary tumour transforming gene 1 (PTTG1) is over-expressed in a variety of endocrine-related tumours. We aimed at evaluatingPTTG1expression and function in human neoplastic parafollicular C-cells, represented by medullary thyroid carcinoma (MTC) and C-cell hyperplasia (CCH) samples and by the TT cell line.Methods: TT cells and tissues derived from human CCH (8 samples) and MTC (12 samples) were analyzed by northern blot, furthermore TT cells were subjected to PTTG gene silencing and cells were analyzed for DNA synthesis.Results:PTTG1expression was significantly higher (p< 0.01) in CCH (3-fold), in papillary thyroid cancer and in MTC (5-fold) than in normal thyroid, and in MTC lymph-node metastases as compared to primary lesions (~2-fold; p < 0.05).PTTG1mRNA and protein correlated with tumour diameter and TNM status (p < 0.05). In TT cells,PTTG1silencing did not completely block DNA synthesis, but significantly reduced [3H]Thymidine incorporation (~50%; p < 0.01) for up to 3 days.Conclusion:PTTG1levels correlate with tumour aggressiveness.PTTG1silencing causes reduced MTC cell proliferation, supporting the hypothesis thatPTTG1might have an important role in C-cell neoplastic proliferation. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 2210-7185 2210-7177 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b3d870cc6fc94f02375d7d26b2a8313Test https://doaj.org/article/fd75fc6faac14c72ad055e2048ebf4b4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5b3d870cc6fc94f02375d7d26b2a8313 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22107185 22107177 |
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