SLAMF1 signaling induces Mycobacterium tuberculosis uptake leading to endolysosomal maturation in human macrophages
| العنوان: | SLAMF1 signaling induces Mycobacterium tuberculosis uptake leading to endolysosomal maturation in human macrophages |
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| المؤلفون: | Angela María Barbero, Luciana Balboa, Federico Fuentes, Verónica E. García, Josefina Celano, Aldana Trotta, Martin A. Estermann, Paula Barrionuevo, Rodrigo Emanuel Hernández Del Pino, Melanie Genoula, Virginia Pasquinelli |
| المصدر: | Journal of Leukocyte Biology. 109:257-273 |
| بيانات النشر: | Oxford University Press (OUP), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Tuberculosis, Adolescent, Endosome, media_common.quotation_subject, CD14, Immunology, Endosomes, Microbiology, Mycobacterium tuberculosis, EEA1, Young Adult, 03 medical and health sciences, 0302 clinical medicine, Immune system, Phagocytosis, Signaling Lymphocytic Activation Molecule Family Member 1, medicine, Humans, Immunology and Allergy, Internalization, Tuberculosis, Pulmonary, Aged, media_common, biology, Macrophages, Cell Biology, Middle Aged, medicine.disease, biology.organism_classification, 030104 developmental biology, 030220 oncology & carcinogenesis, biology.protein, Female, Antibody, Lysosomes, Signal Transduction |
| الوصف: | Tuberculosis dates back to ancient times but it is not a problem of the past. Each year, millions of people die from tuberculosis. After inhalation of infectious droplet nuclei, Mycobacterium tuberculosis reaches the lungs where it can manipulate the immune system and survive within host macrophages, establishing a persistent infection. The signaling lymphocytic activation molecule family member 1 (SLAMF1) is a self-ligand receptor that can internalize gram-negative bacteria and regulate macrophages’ phagosomal functions. In tuberculosis, SLAMF1 promotes Th1-protective responses. In this work, we studied the role of SLAMF1 on macrophages’ functions during M. tuberculosis infection. Our results showed that both M. tuberculosis and IFN-γ stimulation induce SLAMF1 expression in macrophages from healthy donor and Tohoku Hospital Pediatrcs-1 cells. Costimulation through SLAMF1 with an agonistic antibody resulted in an enhanced internalization of M. tuberculosis by macrophages. Interestingly, we found that SLAMF1 interacts with M. tuberculosis and colocalizes with the bacteria and with early and late endosomes/lysosomes markers (EEA1 and LAMP2), suggesting that SLAMF1 recognize M. tuberculosis and participate in the endolysosomal maturation process. Notably, increased levels of SLAMF1 were detected in CD14 cells from pleural effusions of tuberculosis patients, indicating that SLAMF1 might have an active function at the site of infection. Taken together, our results provide evidence that SLAMF1 improves the uptake of M. tuberculosis by human monocyte-derived macrophages. |
| تدمد: | 1938-3673 0741-5400 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a6a44ebbac8b2b2c28beb7e3f15cfafTest https://doi.org/10.1002/jlb.4ma0820-655rrTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....5a6a44ebbac8b2b2c28beb7e3f15cfaf |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19383673 07415400 |
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