Minimal residual disease monitoring via AML1-ETO breakpoint tracing in childhood acute myeloid leukemia
| العنوان: | Minimal residual disease monitoring via AML1-ETO breakpoint tracing in childhood acute myeloid leukemia |
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| المؤلفون: | Meihui Yi, Chao Liu, Xiao-Yan Chen, Xiaofan Zhu, Yongjuan Duan, Wenyu Yang, Min Ruan, Yumei Chen, Ye Guo, Tianyuan Hu, Bingrui Wang, Li Zhang, Xiaojuan Chen, Xuelian Cheng, Yingchi Zhang, Suyu Zong, Tao Cheng, Yao Zou |
| المصدر: | Translational Oncology Translational Oncology, Vol 14, Iss 8, Pp 101119-(2021) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Oncology, Cancer Research, medicine.medical_specialty, Fusion gene, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, hemic and lymphatic diseases, Medicine, Digital polymerase chain reaction, Gene, RC254-282, Original Research, Acute myeloid leukemia, AML1-ETO fusion gene, business.industry, Childhood Acute Myeloid Leukemia, Breakpoint, Myeloid leukemia, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Minimal residual disease, Aml1 eto, body regions, 030104 developmental biology, Droplet digital PCR, 030220 oncology & carcinogenesis, business |
| الوصف: | Highlights l Performed DNA-based ddPCR to accurately monitor the MRD of AE (+) AML. l Identified quantification of the fusion gene correlates strongly with clinical prognosis. Relapse of childhood AML1-ETO (AE) acute myeloid leukemia is the most common cause of treatment failure. Optimized minimal residual disease monitoring methods is required to prevent relapse. In this study, we used next-generation sequencing to identify the breakpoints in the fusion gene and the DNA-based droplet digital PCR (ddPCR) method was used for dynamic monitoring of AE-DNA. The ddPCR technique provides more sensitive and precise quantitation of the AE gene during disease progression and relapse. Quantification of the AE fusion gene by ddPCR further contributes to improved prognosis. Our study provides valuable methods for dynamic surveillance of AE fusion DNA and assistance in determining the prognosis. |
| تدمد: | 1936-5233 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a3f94ddf1cb175ec3660ff8a971705dTest https://pubmed.ncbi.nlm.nih.gov/34000643Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5a3f94ddf1cb175ec3660ff8a971705d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19365233 |
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