Interplay among platelet-activating factor, oxidative stress, and group I metabotropic glutamate receptors modulates neuronal survival
| العنوان: | Interplay among platelet-activating factor, oxidative stress, and group I metabotropic glutamate receptors modulates neuronal survival |
|---|---|
| المؤلفون: | Peimin Zhu, Nicolas G. Bazan, Mark A. DeCoster |
| المصدر: | Journal of Neuroscience Research. 77:525-531 |
| بيانات النشر: | Wiley, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Azoles, Free Radicals, Cell Survival, Platelet Membrane Glycoproteins, Isoindoles, Pharmacology, Receptors, Metabotropic Glutamate, medicine.disease_cause, Neuroprotection, Antioxidants, Receptors, G-Protein-Coupled, Nitric oxide, Rats, Sprague-Dawley, Lactones, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Organoselenium Compounds, Excitatory Amino Acid Agonists, medicine, Animals, Nitric Oxide Donors, Platelet Activating Factor, Cells, Cultured, Neurons, Cell Death, L-Lactate Dehydrogenase, Platelet-activating factor, Superoxide, Neurodegeneration, Vitamin K 3, Long-term potentiation, Hydrogen Peroxide, medicine.disease, Rats, Oxidative Stress, Ginkgolides, Neuroprotective Agents, chemistry, Biochemistry, Metabotropic glutamate receptor, Nerve Degeneration, lipids (amino acids, peptides, and proteins), Diterpenes, Oxidative stress |
| الوصف: | Platelet-activating factor (PAF) is a potent phospholipid messenger in the nervous system that participates in synaptic plasticity and in pathologic processes, including neurodegeneration. Oxidative stress plays important roles in neuronal cell death. To define the interaction between PAF and oxidative radicals in neuronal death, we studied the effects of PAF in the presence of oxidative radicals in primary neurons in culture. Exogenous PAF (50 μM) caused PAF receptor-independent injury to neurons. A nonneurotoxic PAF concentration (500 nM) potentiated neuronal death caused by hydrogen peroxide as determined by lactate dehydrogenase (LDH) assay, Hoechst staining, and TUNEL analysis, but it did not potentiate neuronal death caused by menadione, a superoxide donor, or by the nitric oxide donors 3-morpholino-sydnonimine (SIN-1) and sodium nitroprusside (SNP). This potentiation of the hydrogen peroxide effect was selectively blocked by a PAF membrane-receptor antagonist, BN52021 (5 μM). The neurotoxic effect of PAF and hydrogen peroxide was also completely blocked by ebselen and partially decreased by pretreatment with (S)-3,5-dihydroxyphenylglycine (DHPG), a group I metabotropic glutamate receptor (mGluR) agonist. This study suggests that PAF-receptor antagonists may be useful for neuroprotection. A similar effect might also be obtained with group I mGluR agonists, probably by way of a different underlying mechanism. © 2004 Wiley-Liss, Inc. |
| تدمد: | 1097-4547 0360-4012 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59e21b15dae26807fd203ef0e99cbddcTest https://doi.org/10.1002/jnr.20175Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....59e21b15dae26807fd203ef0e99cbddc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974547 03604012 |
|---|