Functional and phenotypic characterization of CD8+CD28+ and CD28- T cells in atopic individuals sensitized to Dermatophagoides pteronyssinus
| العنوان: | Functional and phenotypic characterization of CD8+CD28+ and CD28- T cells in atopic individuals sensitized to Dermatophagoides pteronyssinus |
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| المؤلفون: | Olga Lourenço, Ana Mafalda Fonseca, Luís Taborda-Barata, Fernando A. Arosa, Artur Paiva |
| المساهمون: | uBibliorum |
| المصدر: | CIÊNCIAVITAE Web of Science Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação instacron:RCAAP |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Hypersensitivity, Immediate, Male, Allergy, Dermatophagoides pteronyssinus, Receptors, Antigen, T-Cell, alpha-beta, CD8+CD28– T cells, CD8-Positive T-Lymphocytes, Lymphocyte Activation, Immunophenotyping, T-Lymphocyte Subsets, Immunology and Allergy, Medicine, Cells, Cultured, education.field_of_study, medicine.diagnostic_test, Atopy, CD28, Receptors, Antigen, T-Cell, gamma-delta, hemic and immune systems, General Medicine, Flow Cytometry, medicine.anatomical_structure, Cytokines, Female, Human, Pulmonary and Respiratory Medicine, Adult, Rhinitis, Allergic, Perennial, T cell, Immunology, Population, Antigen-Presenting Cells, chemical and pharmacologic phenomena, Peripheral blood mononuclear cell, Flow cytometry, Antigen, CD28 Antigens, Animals, Humans, Antigens, Dermatophagoides, education, business.industry, Immunomagnetic Separation, Coculture Techniques, body regions, business, CD8, Muromonab-CD3 |
| الوصف: | Background CD8 + T suppressor cells may play a role in immunoregulation. Recent studies have characterized this population by the lack of the CD28 molecule. These CD8 + CD28 − T cells differ phenotypically and functionally from CD8 + CD28 + T cells. Little is known about CD8 + CD28 – cells in atopy. Our aim was to analyze the phenotype and functional properties of CD8 + CD28 − T cells in atopic and non-atopic individuals. Methods Peripheral blood mononuclear cells (PBMC) were obtained after density gradient centrifugation. CD8 + CD28 − and CD8 + CD28 + T cells were isolated using immunomagnetic beads. Relative percentagesof these cells and expression of several phenotypic markers were analyzed by flow cytometry. Proliferation was assessed by thymidine incorporation in isolated populations and in co-cultures with PBMC using Dermatophagoides pteronyssinus as stimulus. Cytokine synthesis was evaluated in culture supernatants by cytometric bead array. Results The relative percentages of CD8 + CD28 − T cells and their phenotypic expression in atopic and non-atopic volunteers were not significantly different. However, CD8 + CD28 − T cells showed greater proliferation than did CD8 + CD28 + T cells when stimulatedwith D. pteronyssinus , although cytokine synthesis patterns were similar. CD8 + CD28 − co-cultures with PBMC showed greater proliferation than CD8 + CD28 + T cell co-cultures, but cytokine synthesis patterns were not different. Conclusions Our data confirm phenotypic and functional differences between CD28 + and CD28 − T cells, irrespective of atopic status. Purified human CD8 + CD28 − T cells, freshly isolated from peripheral blood, do not have suppressor properties on allergen- specific proliferation or on cytokine synthesis in PBMC. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::596fa207771087d7e36910530fb01a46Test https://hdl.handle.net/10400.6/7473Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....596fa207771087d7e36910530fb01a46 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |