MicroRNA ‐mediated extracellular matrix remodeling in squamous cell carcinoma of the oral cavity
| العنوان: | |
|---|---|
| المؤلفون: | Marina de Menezes Ishikawa, Gilberto Mendes Menderico Junior, Raquel Ajub Moyses, Evandro Sobroza de Mello, Nayara Stephânie Sousa Santos, Thérèse Rachell Theodoro, Rogério Aparecido Dedivitis, Luiz Paulo Kowalski, Fatima Solange Pasini, Claudio Roberto Cernea, Leandro Luongo de Matos, Marco Aurélio Vamondes Kulcsar, Maria Aparecida da Silva Pinhal |
| المصدر: | Head & Neck. |
| بيانات النشر: | Wiley, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Extracellular matrix component, Malignancy, medicine.disease_cause, Extracellular matrix, 03 medical and health sciences, Type IV collagen, 0302 clinical medicine, Cell Line, Tumor, Tumor Microenvironment, medicine, Carcinoma, Humans, Cell Proliferation, Retrospective Studies, Basement membrane, Tumor microenvironment, business.industry, medicine.disease, Extracellular Matrix, Gene Expression Regulation, Neoplastic, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, Otorhinolaryngology, 030220 oncology & carcinogenesis, Carcinoma, Squamous Cell, Cancer research, Mouth Neoplasms, business, Carcinogenesis |
| الوصف: | Background: It has been demonstrated that for oral cavity squamous cell carcinoma (SCC) as tumor thickness increases, there are lower rates of survival. Several molecular factors have been studied to determine this invasive behavior, including microRNAs, that play a role in the tumor microenvironment. Methods: The aim of this study is evaluate the role of extracellular matrix remodeling, as well as the involvement of microRNAs, in the process of oral cavity SCC carcinogenesis. This was a retrospective study with patients operated on for SCC of the oral cavity, in addition to a group of oral mucosa samples from paired patients. RNA extraction was performed, followed by complementary DNA amplification of microRNAs related to adhesion, migration, cell proliferation, apoptosis, and constituents of the extracellular matrix. We also performed immunohistochemical reactions for markers involved in the same biological processes. Results: High expression of miR-21-5p and miR-106-5p and low expression of miR-320a and miR-222-3p were predictors of malignancies and mir21-5p, individually, showed the best differentiation between the groups (AUC = 0.972). Regarding the immunohistochemical markers, there was greater expression of p53, EGFR, metalloproteinase-2 (MMP-2), laminin beta, Ki-67 and CD34 in the tumor cells than in the healthy mucosa. Furthermore, increased expression of MMP-2, metalloproteinase-9 (MMP-9), laminin alpha and laminin beta in tumor-related fibroblasts and lower continuity of type IV collagen in the basement membrane were observed. Conclusions: These results demonstrate the biological effects of microRNAs on the carcinogenesis of SCC of the oral cavity as well as the intense modification of the tumor microenvironment. |
| تدمد: | 1097-0347 1043-3074 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::590c6224350179ce4fe5219efa934c67Test https://doi.org/10.1002/hed.26686Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....590c6224350179ce4fe5219efa934c67 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970347 10433074 |
|---|