IL-10+ NK and TGF-β+ NK cells play negative regulatory roles in HIV infection
| العنوان: | IL-10+ NK and TGF-β+ NK cells play negative regulatory roles in HIV infection |
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| المؤلفون: | Jing Liu, Shi Qian, Yajing Fu, Zining Zhang, Hong Shang, Junjie Xu, Xiaowan Yin, Xi Chen, Meichen Ma, Yongjun Jiang, Xiaojuan Sun, Xiaoxu Han, Mei Yang |
| المصدر: | BMC Infectious Diseases, Vol 18, Iss 1, Pp 1-10 (2018) BMC Infectious Diseases |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, Male, 0301 basic medicine, Chemokine, HIV Infections, Granzymes, 0302 clinical medicine, Transforming Growth Factor beta, Cytotoxic T cell, Medicine, Interferon gamma, IFN-γ, Cells, Cultured, biology, Interleukin, Recombinant Proteins, Interleukin-10, Killer Cells, Natural, Interleukin 10, Infectious Diseases, Anti-Retroviral Agents, IL-10, RNA, Viral, Research Article, medicine.drug, Adult, TGF-β, Immune regulation, NK, lcsh:Infectious and parasitic diseases, Interferon-gamma, Young Adult, 03 medical and health sciences, Lysosomal-Associated Membrane Protein 1, Humans, lcsh:RC109-216, Perforin, business.industry, HIV, CD4 Lymphocyte Count, Granzyme B, Antiretroviral treatment, 030104 developmental biology, Granzyme, Case-Control Studies, Immunology, Leukocytes, Mononuclear, biology.protein, business, 030215 immunology |
| الوصف: | Background Natural killer (NK) cells play cytotoxic roles by targeting tumor cells or virus infected cells, they also play regulatory roles by secreting cytokines and chemokines. Transforming growth factor (TGF)-β and interleukin (IL)-10 are important immunosuppressive cytokines potentially related to the immune dysregulation that occurs in the infection of human immunodeficiency virus (HIV). NK cells are an important source of TGF-β and a main early producer of IL-10 in response to viral infection. Here, we evaluated the percentages of IL-10+ and TGF-β+ NK cells in HIV-infected patients relative to healthy controls (HCs). Methods Study participants (n = 63) included 31 antiretroviral treatment (ART)-naïve HIV-infected patients, 17 ART-treated HIV-infected patients, and 15 HIV-negative HCs. Expression of IL-10 or TGF-β in NK cells was examined by flow cytometry, and the influences of recombinant IL-10 (rIL-10) or recombinant TGF-β (rTGF-β) on NK cell function were investigated in vitro. Results Compared with HCs, ART-naïve HIV-infected patients had increased percentages of IL-10+ (2.0% vs. 0.4%, p = 0.015) and TGF-β+ (4.5% vs. 2.1%, p = 0.022) NK cells, and ART-treated patients also had a higher percentage of IL-10+ NK cells (2.5% vs. 0.4%, p = 0.002). The percentages of IL-10+ and TGF-β+ NK cells were positively correlated (r = 0.388; p = 0.010). The results of in vitro experiments demonstrated that rIL-10 and rTGF-β inhibited NK cell CD107a expression (p = 0.037 and p = 0.024, respectively), IFN-γ secretion (p = 0.006, p = 0.016, respectively), and granzyme B release after stimulation (p = 0.014, p = 0.040, respectively). Conclusions Our data suggest that the percentages of IL-10+ or TGF-β+ NK cells are increased in HIV-infected patients, and that rIL-10 and/or rTGF-β can inhibit NK cell functions in vitro, providing a potential therapeutic target for strategies aimed at combating HIV infection. |
| تدمد: | 1471-2334 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5884ecc53df10690cfcd9fec44a86a60Test https://doi.org/10.1186/s12879-018-2991-2Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5884ecc53df10690cfcd9fec44a86a60 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712334 |
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