CHCHD2 is a potential prognostic factor for NSCLC and is associated with HIF-1a expression
| العنوان: | CHCHD2 is a potential prognostic factor for NSCLC and is associated with HIF-1a expression |
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| المؤلفون: | Xin Yin, Zhipei Zhang, Ying Sun, Jinghua Xia |
| المصدر: | BMC Pulmonary Medicine, Vol 20, Iss 1, Pp 1-11 (2020) BMC Pulmonary Medicine |
| بيانات النشر: | BMC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Pulmonary and Respiratory Medicine, Adult, Male, Lung Neoplasms, CHCHD2, Cell, HIF-1α, Prognostic, Metastasis, 03 medical and health sciences, Mice, 0302 clinical medicine, Non-small cell lung cancer, Carcinoma, Non-Small-Cell Lung, Biomarkers, Tumor, Medicine, Animals, Humans, Survival analysis, 030304 developmental biology, Aged, Neoplasm Staging, Aged, 80 and over, lcsh:RC705-779, 0303 health sciences, business.industry, Proportional hazards model, Hazard ratio, lcsh:Diseases of the respiratory system, Hypoxia (medical), Middle Aged, medicine.disease, Hypoxia-Inducible Factor 1, alpha Subunit, Prognosis, Immunohistochemistry, Survival Analysis, Blot, DNA-Binding Proteins, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Lymphatic Metastasis, Cancer research, Female, medicine.symptom, business, Research Article, Transcription Factors |
| الوصف: | Background CHCHD2 was identified a novel cell migration-promoting gene, which could promote cell migration and altered cell adhesion when ectopically overexpressed in NIH3T3 fibroblasts, and it was identified as a protein necessary for OxPhos function as well. However, the clinic relevance of CHCHD2 expression in NSCLC remains unclear. Here we assumed that CHCHD2 expression would accompanies the expression of HIF-1α to response hypoxia in the occurrence of NSCLC. Methods In order to verify this hypothesis, correlations among the expression levels of CHCHD2 and HIF-1α were detected and analyzed in 209 pair cases of NSCLC. The expression and location of these molecules were assessed using Immunohistochemistry, immunohistofluorescence, qRT-PCR and western blotting. The differences and correlations of the expression of these two molecules with clinical pathological characteristics in NSCLC were statistically analyzed using Wilcoxon (W) text, Mann-Whitney U, Kruskal-Wallis H and cross-table tests. Kaplan-Meier survival analysis and Cox proportional hazards models were used to estimate the effect of the expression of CHCHD2 and HIF-1α on the patients’ survival. Results Data showed that CHCHD2 and HIF-1α expression were higher in NSCLC than in normal tissues (all P = 0.000). CHCHD2 expression was significantly related with smoking, tumor size, differentiation degree, TNM Stage, lymph metastasis (all P<0.05). The HIF-1α expression was significantly associated with smoking, tumor category, differentiation degree, TNM Stage, Lymph metastasis (all P<0.05). There was a marked correlation of CHCHD2 and HIF-1α expression with histological type, differentiation and lymph metastasis of NSCLC (all P<0.05, rs>0.3). Immunohistofluorescence showed that there were co-localization phenomenon in cytoplasm and nucleus between CHCHD2 and HIF-1α expression. NSCLC patients with higher CHCHD2 and HIF-1α expression had a significantly worse prognosis than those with lower CHCHD2 and HIF-1α expression (all P = 0.0001; log-rank test). The multivariate analysis indicated that CHCHD2 expression was an independent prognostic factor in NSCLC (hazard ratio [HR], 0.492, P = 0.001). Conclusion Our results indicate that over-expression of CHCHD2 would promote the expression of HIF-1α to adapt the hypoxia microenviroment in NSCLC and CHCHD2 could serves as a prognostic biomarker in NSCLC. |
| اللغة: | English |
| تدمد: | 1471-2466 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57fbb488b6207d785aba1ad550e349b4Test https://doaj.org/article/002b0c468859456f9262068df95cd277Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....57fbb488b6207d785aba1ad550e349b4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712466 |
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