Shu complex SWS1-SWSAP1 promotes early steps in mouse meiotic recombination
| العنوان: | Shu complex SWS1-SWSAP1 promotes early steps in mouse meiotic recombination |
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| المؤلفون: | Abreu, Carla M., Prakash, Rohit, Romanienko, Peter J., Roig, I., Keeney, Scott, Jasin, Maria, Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia |
| المصدر: | Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018) Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Recercat. Dipósit de la Recerca de Catalunya instname Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) Nature Communications |
| بيانات النشر: | Nature Publishing Group, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, RAD51, General Physics and Astronomy, Cell Cycle Proteins, Chromosomal crossover, Chromosome segregation, 0302 clinical medicine, Crossing Over, Genetic, lcsh:Science, Recombination, Genetic, Multidisciplinary, Nuclear Proteins, Cell cycle proteins, Rad51 recombinase, Spermatozoa, Cell biology, Meiosis, Female, Science, Biology, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, Nuclear proteins, Shu complex, Animals, BRCA2 Protein, Base Sequence, urogenital system, fungi, Chromosome pairing, General Chemistry, DNA, G2-M DNA damage checkpoint, Phosphate-Binding Proteins, Base sequence, Mice, Mutant Strains, Mice, Inbred C57BL, Checkpoint Kinase 2, Chromosome Pairing, 030104 developmental biology, Crossing over, genetic, Infertility, Mice, Mutant strains, Mutation, DMC1, lcsh:Q, Rad51 Recombinase, BRCA2 protein, Homologous recombination, 030217 neurology & neurosurgery, Checkpoint kinase 2 |
| الوصف: | The DNA-damage repair pathway homologous recombination (HR) requires factors that promote the activity of strand-exchange protein RAD51 and its meiosis-specific homolog DMC1. Here we show that the Shu complex SWS1-SWSAP1, a candidate for one such HR regulator, is dispensable for mouse viability but essential for male and female fertility, promoting the assembly of RAD51 and DMC1 on early meiotic HR intermediates. Only a fraction of mutant meiocytes progress to form crossovers, which are crucial for chromosome segregation, demonstrating crossover homeostasis. Remarkably, loss of the DNA damage checkpoint kinase CHK2 rescues fertility in females without rescuing crossover numbers. Concomitant loss of the BRCA2 C terminus aggravates the meiotic defects in Swsap1 mutant spermatocytes, suggesting an overlapping role with the Shu complex during meiotic HR. These results demonstrate an essential role for SWS1-SWSAP1 in meiotic progression and emphasize the complex interplay of factors that ensure recombinase function. Homologous recombination ensures genome integrity during meiotic recombination. Here the authors reveal that factors SWS1 and SWSAP1 are critical for meiotic homologues recombination, particularly in promoting assembly of RAD51 and DMC1 on early recombination intermediates. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2041-1723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57c91dfdd309a7d166e0ccd94e2b933dTest http://link.springer.com/article/10.1038/s41467-018-06384-xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....57c91dfdd309a7d166e0ccd94e2b933d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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