G-fibre cell wall development in willow stems during tension wood induction
| العنوان: | G-fibre cell wall development in willow stems during tension wood induction |
|---|---|
| المؤلفون: | Yongfang Wan, Angela Karp, Steven J. Hanley, Rowan A. C. Mitchell, Peter R. Shewry, C. Gritsch |
| المصدر: | Journal of Experimental Botany |
| بيانات النشر: | Oxford University Press (OUP), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0106 biological sciences, Physiology, Plant Science, 7. Clean energy, 01 natural sciences, Cell Wall, Gene Expression Regulation, Plant, (1–4)-β-D-galactan, In Situ Hybridization, Plant Proteins, chemistry.chemical_classification, 0303 health sciences, Microscopy, Confocal, biology, mannan, Salix, CCRC-M38, Wood, Cellulose microfibril, gelatinous fibres, Fiber cell, Secondary cell wall, Research Paper, fasciclin, Willow, reaction wood, xylan, Polysaccharide, LM5, Cell wall, 03 medical and health sciences, Microscopy, Electron, Transmission, Polysaccharides, Xylem, homogalacturonan, Botany, immunofluorescence, LM10, 030304 developmental biology, SxCOBL4, willow, biology.organism_classification, Xylan, immunogold, tension wood, chemistry, SxFLA12, TEM, LM21, 010606 plant biology & botany |
| الوصف: | Highlight Immunolocalization of cell wall polysaccharides in gelatinous fibres of willow tension wood indicates a distinct distribution with a particular enrichment of (1–4)-β-D-galactan in the G-layer. Willows (Salix spp.) are important as a potential feedstock for bioenergy and biofuels. Previous work suggested that reaction wood (RW) formation could be a desirable trait for biofuel production in willows as it is associated with increased glucose yields, but willow RW has not been characterized for cell wall components. Fasciclin-like arabinogalactan (FLA) proteins are highly up-regulated in RW of poplars and are considered to be involved in cell adhesion and cellulose biosynthesis. COBRA genes are involved in anisotropic cell expansion by modulating the orientation of cellulose microfibril deposition. This study determined the temporal and spatial deposition of non-cellulosic polysaccharides in cell walls of the tension wood (TW) component of willow RW and compared it with opposite wood (OW) and normal wood (NW) using specific antibodies and confocal laser scanning microscopy and transmission electron microscopy. In addition, the expression patterns of an FLA gene (SxFLA12) and a COBRA-like gene (SxCOBL4) were compared using RNA in situ hybridization. Deposition of the non-cellulosic polysaccharides (1–4)-β-D-galactan, mannan and de-esterified homogalacturonan was found to be highly associated with TW, often with the G-layer itself. Of particular interest was that the G-layer itself can be highly enriched in (1–4)-β-D-galactan, especially in G-fibres where the G-layer is still thickening, which contrasts with previous studies in poplar. Only xylan showed a similar distribution in TW, OW, and NW, being restricted to the secondary cell wall layers. SxFLA12 and SxCOBL4 transcripts were specifically expressed in developing TW, confirming their importance. A model of polysaccharides distribution in developing willow G-fibre cells is presented. |
| وصف الملف: | application/pdf |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::577a1b7ab379bdd50a6b97423d6202cfTest https://repository.rothamsted.ac.uk/download/555f380bc0afce78fc210a7c1410a6cb9bdfb039b2f7621993dbbdd7111f3bfb/13809387/erv358.pdfTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....577a1b7ab379bdd50a6b97423d6202cf |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |