Phenotypic and genotypic drug sensitivity profiles of Mycobacterium tuberculosis infection and associated factors in northeastern Ethiopia
| العنوان: | Phenotypic and genotypic drug sensitivity profiles of Mycobacterium tuberculosis infection and associated factors in northeastern Ethiopia |
|---|---|
| المؤلفون: | Berhanu Erko, Balako Gumi, Fikru Gashaw, Gobena Ameni, Yalemtsehay Mekonnen, Bazezew Yenew, Misikir Amare |
| المصدر: | BMC Infectious Diseases BMC Infectious Diseases, Vol 21, Iss 1, Pp 1-12 (2021) |
| بيانات النشر: | Research Square Platform LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Veterinary medicine, Tuberculosis, Adolescent, Genotype, Antitubercular Agents, Drug resistance, Microbial Sensitivity Tests, lcsh:Infectious and parasitic diseases, Mycobacterium tuberculosis, Young Adult, Medical microbiology, Drug Resistance, Bacterial, medicine, Isoniazid, Humans, lcsh:RC109-216, Aged, biology, BACTEC MGIT 960, Gold standard (test), Middle Aged, biology.organism_classification, medicine.disease, Infectious Diseases, Cross-Sectional Studies, Phenotype, Parasitology, Female, Northeastern Ethiopia, Ethiopia, Reagent Kits, Diagnostic, Rifampin, Rifampicin, medicine.drug, Mycobacterium, Research Article, MTBDRplus assay |
| الوصف: | Background Tuberculosis is a devastating and a deadly disease despite the novel advances in its diagnostic tools and drug therapy. Drug resistant Mycobacterium contributes a great share to tuberculosis mortality. Status of drug resistance and patients’ awareness toward the disease is unknown in northeastern Ethiopia. Thus, the aim of this study was to determine the phenotypic and genotypic drug sensitivity patterns and associated factors in Oromia Special Zone and Dessie Town, northeastern Ethiopia. Methods In a cross-sectional study, 384 smear positive tuberculosis cases were recruited and Löwenstein-Jensen culture was done. The performance of GenoTypic MTBDRplus assay using the conventional BACTEC MGIT 960 as a “gold standard” was determined. Drug resistant strains were identified using spoligotyping. Pearson Chi-square test was used to determine the association of drug sensitivity test and tuberculosis type, lineages, dominant strains and clustering of the isolates. Results The 384 smear positive Mycobacterium samples were cultured on LJ media of which 29.2% (112/384) as culture positive. A fair agreement was found between MTBDRplus assay and the conventional MGIT test in detecting the Mycobacterium tuberculosis with sensitivity, specificity, positive and negative predictive value of 94.2, 30.2, 68.4 and 76.5%, respectively. Among LJ culture positive samples 95 of them gave valid result for MTBDRplus assay and 16.8% (16/95) as drug resistant. Similarly, MGIT subculture was made for the 112 isolates and 69 of them gave positive result with 15.9% (11/69) as drug resistant. Cohen’s kappa value showed almost a perfect agreement between the two testing methods in detecting rifampicin (sensitivity 100% and specificity 98.3%) and multi-drug resistance (sensitivity 83.3% and specificity 100%). Spoligotyping identified 76.5% (13/17) of the drug resistant isolates as Euro-American and family 33 as the predominant family. Significant association was observed between drug resistant isolates and the dominant strains (χ2: 34.861; p = 0.040) of the Mycobacterium. Conclusion Higher magnitude of drug resistance was found in the study area. The GenoTypic MDRTBplus assay had an acceptable drug sensitivity testing performance. |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57510ec679eb4f845ffde7d3024b9a69Test https://doi.org/10.21203/rs.3.rs-127306/v1Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....57510ec679eb4f845ffde7d3024b9a69 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |