Protective effect of 3-hydroxybutyrate against endoplasmic reticulum stress-associated vascular endothelial cell damage induced by low glucose exposure
| العنوان: | Protective effect of 3-hydroxybutyrate against endoplasmic reticulum stress-associated vascular endothelial cell damage induced by low glucose exposure |
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| المؤلفون: | Yuji Tajiri, Satomi Kakino, Tsuyoshi Ohki, Yayoi Kurita, Kentaro Yamada, Hitomi Nakayama, Xiaohong Yuan, Kento Hara, Eri Soejima, Kayo Tanaka |
| المصدر: | PLoS ONE PLoS ONE, Vol 13, Iss 3, p e0191147 (2018) |
| بيانات النشر: | Public Library of Science, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, X-Box Binding Protein 1, lcsh:Medicine, Gene Expression, Apoptosis, Endoplasmic Reticulum, Biochemistry, Epithelium, eIF-2 Kinase, 0302 clinical medicine, Animal Cells, Medicine and Health Sciences, lcsh:Science, Caspase, Cells, Cultured, Caspase 7, Caspase 8, Multidisciplinary, Secretory Pathway, biology, Cell Death, 3-Hydroxybutyric Acid, Chemistry, Organic Compounds, Caspase 3, Monosaccharides, Ketones, Endoplasmic Reticulum Stress, Caspase Inhibitors, Endothelial stem cell, Cell Processes, Physical Sciences, Cellular Structures and Organelles, Cellular Types, Anatomy, Research Article, medicine.medical_specialty, XBP1, Nitric Oxide Synthase Type III, Cell Survival, Carbohydrates, Protective Agents, 03 medical and health sciences, Internal medicine, DNA-binding proteins, medicine, Genetics, Human Umbilical Vein Endothelial Cells, Humans, Gene Regulation, Viability assay, RNA, Messenger, Protein kinase A, L-Lactate Dehydrogenase, ATF6, Endoplasmic reticulum, lcsh:R, Organic Chemistry, Chemical Compounds, Biology and Life Sciences, Endothelial Cells, Proteins, Epithelial Cells, Cell Biology, Hypoglycemia, Regulatory Proteins, Activating Transcription Factor 6, 030104 developmental biology, Endocrinology, Glucose, Biological Tissue, Metabolic Disorders, biology.protein, Unfolded protein response, lcsh:Q, Acids, 030217 neurology & neurosurgery, Transcription Factors |
| الوصف: | Aims/hypothesis The aim of this study was to elucidate the mechanism by which severe hypoglycemia accelerates vascular complications. Furthermore, we assessed the possible protective effect of ketone bodies against the endothelial cell damage caused by glucose deficiency. Methods Human umbilical vein endothelial cells (HUVECs) were cultured at a glucose level of either 0.56 or 5.6 mmol/L with or without 3-hydroxybutyrate (3-HB) supplementation. Cell viability was assessed with a CCK-8 assay and a lactate dehydrogenase (LDH) release assay. The activity of caspases was measured using fluorogenic substrates. The expression of genes associated with endothelial cell function and endoplasmic reticulum (ER) stress was evaluated by real-time quantitative PCR. Protein levels of ER stress-related molecules were assessed by Western blotting. Results Culture of HUVECs in low-glucose medium for 24 or 48 h resulted in reduction of cell viability accompanied by activation of caspase-3/7 and caspase-8. The addition of a pan caspase inhibitor attenuated the cell death. After incubation in the low-glucose medium, we found reduced mRNA and protein levels of endothelial nitric oxide synthase. ER stress responses mediated by phosphorylation of protein kinase RNA-like ER kinase (PERK) and cleavage of activating transcription factor 6 (ATF6) were augmented, but X-box binding protein 1 (Xbp1) splicing was reduced. Most of these responses to glucose deficiency were significantly attenuated by supplementation with 3-HB. Conclusions/interpretation These observations showed that exposure to low glucose induces ER stress, caspase activation, endothelial cell dysfunction and cell death. The beneficial effects of 3-HB shown in this study suggest that hypoketonemic severe hypoglycemia induced by insulin injections or insulin secretagogue administration may be more harmful than hyperketonemic severe hypoglycemia. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57196edcd6386b18b5e3c78a24917406Test http://europepmc.org/articles/PMC5858752Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....57196edcd6386b18b5e3c78a24917406 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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