Nod Factor Effects on Root Hair-Specific Transcriptome of Medicago truncatula: Focus on Plasma Membrane Transport Systems and Reactive Oxygen Species Networks
| العنوان: | Nod Factor Effects on Root Hair-Specific Transcriptome of Medicago truncatula: Focus on Plasma Membrane Transport Systems and Reactive Oxygen Species Networks |
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| المؤلفون: | Vincent Maillol, Alexandre Boscari, Etienne Danchin, Eric Samain, Isabelle Damiani, Bruno Touraine, Jean-Marie Frachisse, Alain Puppo, Véronique Brunaud, Isabelle Gaillard, Sylvain Cottaz, Alice Drain, Martine Da Rocha, Jean-Christophe Boyer, Hervé Sentenac, Hatem Rouached, Marjorie Guichard, Corinne Rancurel, Sandrine Balzergue, Sébastien Fort, Yanhua Su, Nicolas Pauly, Julien Thouin, Cécile Fizames |
| المساهمون: | Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institute for Integrative Biology of the Cell, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherches sur les Macromolécules Végétales (CERMAV ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Méthodes et Algorithmes pour la Bioinformatique (MAB), Laboratoire d'Informatique de Robotique et de Microélectronique de Montpellier (LIRMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), State Key Laboratory of Soil and Sustainable Agriculture, Chinese Academy of Sciences [Beijing] (CAS)-Institute of Soil Science, Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Centre de Recherches sur les Macromolécules Végétales (CERMAV), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Pauly, Nicolas, Sentenac, Herve, Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut de Biologie Intégrative de la Cellule (I2BC) |
| المصدر: | Frontiers in Plant Science Frontiers in Plant Science, Frontiers, 2016, 7, pp.794. ⟨10.3389/fpls.2016.00794⟩ Frontiers in Plant Science, Frontiers, 2015, 7, pp.794. ⟨10.3389/fpls.2016.00794⟩ Frontiers in Plant Science (7), 794. (2016) Frontiers in Plant Science, Vol 7 (2016) Frontiers in Plant Science, 2016, 7, pp.794. ⟨10.3389/fpls.2016.00794⟩ |
| بيانات النشر: | Frontiers Media SA, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Plant Science, lcsh:Plant culture, racine aérienne, Root hair, 01 natural sciences, root hairs, Nod factor, Transcriptome, Nod factors (lipochitooligosaccharides), 03 medical and health sciences, reactive oxygen species, Symbiosis, symbiose rhizobium legumineuse, Medicago truncatula, Botany, Rhizobial Symbiosis, Root Hairs, Plasma Membrane Transpor, Reactive Oxygen, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, lcsh:SB1-1110, transport membranaire, Gene, Original Research, Calcium signaling, Vegetal Biology, biology, Membrane transport, deep-RNA sequencing, biology.organism_classification, Cell biology, legume-rhizobium symbiosis, 030104 developmental biology, plasma membrane transport systems, Biologie végétale, 010606 plant biology & botany |
| الوصف: | International audience; Root hairs are involved in water and nutrient uptake, and thereby in plant autotrophy. In legumes, they also play a crucial role in establishment of rhizobial symbiosis. To obtain a holistic view of Medicago truncatula genes expressed in root hairs and of their regulation during the first hours of the engagement in rhizobial symbiotic interaction, a high throughput RNA sequencing on isolated root hairs from roots challenged or not with lipochitooligosaccharides Nod factors (NF) for 4 or 20 h was carried out. This provided a repertoire of genes displaying expression in root hairs, responding or not to NF, and specific or not to legumes. In analyzing the transcriptome dataset, special attention was paid to pumps, transporters, or channels active at the plasma membrane, to other proteins likely to play a role in nutrient ion uptake, NF electrical and calcium signaling, control of the redox status or the dynamic reprogramming of root hair transcriptome induced by NF treatment, and to the identification of papilionoid legume-specific genes expressed in root hairs. About 10% of the root hair expressed genes were significantly up- or down-regulated by NF treatment, suggesting their involvement in remodeling plant functions to allow establishment of the symbiotic relationship. For instance, NF-induced changes in expression of genes encoding plasma membrane transport systems or disease response proteins indicate that root hairs reduce their involvement in nutrient ion absorption and adapt their immune system in order to engage in the symbiotic interaction. It also appears that the redox status of root hair cells is tuned in response to NF perception. In addition, 1176 genes that could be considered as "papilionoid legume-specific" were identified in the M. truncatula root hair transcriptome, from which 141 were found to possess an ortholog in every of the six legume genomes that we considered, suggesting their involvement in essential functions specific to legumes. This transcriptome provides a valuable resource to investigate root hair biology in legumes and the roles that these cells play in rhizobial symbiosis establishment. These results could also contribute to the long-term objective of transferring this symbiotic capacity to non-legume plants. |
| وصف الملف: | application/pdf |
| تدمد: | 1664-462X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55bda4083bc56f8e6c883b57141020d3Test https://doi.org/10.3389/fpls.2016.00794Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....55bda4083bc56f8e6c883b57141020d3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1664462X |
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