Antileukemic Effects of Anti-miR-146a, Anti-miR-155, Anti-miR-181a, and Prednisolone on Childhood Acute Lymphoblastic Leukemia
| العنوان: | Antileukemic Effects of Anti-miR-146a, Anti-miR-155, Anti-miR-181a, and Prednisolone on Childhood Acute Lymphoblastic Leukemia |
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| المؤلفون: | Ozgur Cogulu, Burak Durmaz, Araz Alpay, Bakiye Goker Bagca, Serap Aksoylar, Cumhur Gündüz, Sunde Yilmaz Susluer |
| المصدر: | BioMed Research International BioMed Research International, Vol 2021 (2021) |
| بيانات النشر: | Hindawi Limited, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Apoptosis, IC50, microRNA 181a, gene silencing, genetics, antineoplastic agent, B-Lymphocytes, microRNA, drug cytotoxicity, drug effect, Cell Cycle, prednisolone, General Medicine, Transfection, cell line, Cell cycle, protein function, Precursor Cell Lymphoblastic Leukemia-Lymphoma, unclassified drug, BrdU assay, Leukemia, Prednisolone, Medicine, Research Article, medicine.drug, MIrn181 microRNA, human, Article Subject, Antineoplastic Agents, acute lymphoblastic leukemia, RNA antibody, WST-1 assay, General Biochemistry, Genetics and Molecular Biology, Article, miR-155, mitochondrial membrane potential, Cell Line, Tumor, medicine, gene expression profiling, Humans, controlled study, human, procedures, Childhood Acute Lymphoblastic Leukemia, cell viability, Cell Proliferation, General Immunology and Microbiology, B lymphocyte, business.industry, MIRN146 microRNA, human, human cell, microRNA 146a, trypan blue assay, tumor cell line, medicine.disease, drug efficacy, antileukemic activity, MicroRNAs, Cancer research, microRNA 155, genetic transfection, business, metabolism, MIRN155 microRNA, human |
| الوصف: | Prednisolone has been used frequently in the treatment of acute lymphoblastic leukemia. However, to overcome the challenges of the treatment, the development of additional therapies is of great importance. Small, non-protein-coding RNAs, namely, microRNAs (miRNAs), are critical epigenetic regulators with physiological and pathological importance. This study is aimed at determining the effects of miR-146a, miR-155, and miR-181a inhibition with their corresponding anti-miRs on both leukemic and healthy cells, individually and with prednisolone. Leukemic (SUP-B15) and healthy B-lymphocyte (NCI-BL 2171) cell lines were used in this study. A total of 12 experimental groups included individual and combinational silenced ALL-associated miRNAs (hsa-miR-155, hsa-miR-146a, and hsa-miR-181a) and their combination with prednisolone. Cytotoxicity, proliferation, cell cycle, and apoptosis analyses were performed by using WST-1, trypan blue, APC-BrdU, Annexin V, and JC-1 methods in each study group, respectively. To control the effectiveness of anti-miR transfection and prednisolone application, miRNA expression analysis was performed from all groups. Anti-miR application was effective on the viability, proliferation, cell cycle, and apoptosis of leukemia cells, and this effect was increased with prednisolone administration. In addition, this activity was found to be very low on healthy cells. In conclusion, anti-miR applications may have the potential for clinical use of adjuvant to or as an alternative to conventional therapies for childhood acute lymphoblastic leukemia. © 2021 Burak Durmaz et al. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::554c05eafe2669862afa4781d9c23cc5Test https://hdl.handle.net/11454/79720Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....554c05eafe2669862afa4781d9c23cc5 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |