Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient
| العنوان: | Case report: histopathology and molecular pathology analysis on enteric tissue of a COVID-19 patient |
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| المؤلفون: | Yanling Feng, Jilin Cheng, Yuhan Shi, Wenjuan Guo, Tangkai Qi, Feng Li, Hongzhou Lu, Qingguo Wu, Yun Ling, Jingjing Xu, Yuexiang Yang, Shu Song, Lvyin Hu, Dong Zeng |
| المصدر: | Diagnostic Pathology, Vol 16, Iss 1, Pp 1-6 (2021) Diagnostic Pathology |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Pathology, medicine.medical_specialty, Histology, Gastrointestinal Diseases, viruses, H&E stain, In situ hybridization, Grocott's methenamine silver stain, Stain, Pathology and Forensic Medicine, law.invention, COVID-19 Testing, Intestinal mucosa, law, Case report, Medicine, Humans, RB1-214, Intestinal Mucosa, Aged, business.industry, SARS-CoV-2, Macrophages, virus diseases, COVID-19, General Medicine, Immunohistochemistry, Staining, Gram staining, RNA, Viral, business, Macrophage proliferation, Biomarkers |
| الوصف: | Aims Patients with COVID-19 can also have enteric symptoms. Here we analyzed the histopathology of intestinal detachment tissue from a patient with COVID-19. Methods The enteric tissue was examined by hematoxylin & eosin stain, PAS (Periodic acid–Schiff) staining, Gram staining, Ziehl–Neelsen stain and Grocott’s Methenamine Silver (GMS) Stain. The distribution of CD3, CD4, CK20 and CD68, cytomegalovirus (CMV) and Herpes Simplex Virus (HSV) antigen were determined by immunohistochemistry. In situ hybridization (ISH) of SARS-CoV-2 and Epstein-Barr virus-encoded small RNA (EBER) were also performed. Results We observed mucosal epithelium shedding, intestinal mucosal erosion, focal inflammatory necrosis with hemorrhage, massive neutrophil infiltration, macrophage proliferation accompanied by minor lymphocyte infiltration. Fungal spores and gram positive cocci but not mycobacteria tuberculosis were identified. Immunohistochemistry staining showed abundant CD68+ macrophages but few lymphocytes infiltration. HSV, CMV and EBV were negative. ISH of SARS-CoV-2 RNA showed positive signal which mostly overlapped with CD68 positivity. Conclusions The in situ detection of SARS-CoV-2 RNA in intestinal macrophages implicates a possible route for gastrointestinal infection. Further study is needed to further characterize the susceptibility of enteric cells to SARS-CoV-2 infection. |
| اللغة: | English |
| تدمد: | 1746-1596 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54721fc31262001e48d9108d9c1244c3Test https://doaj.org/article/d3ed447a5805401e914a2616b47e8c06Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....54721fc31262001e48d9108d9c1244c3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17461596 |
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