Global Analysis of Salmonella Alternative Sigma Factor E on Protein Translation
| العنوان: | Global Analysis of Salmonella Alternative Sigma Factor E on Protein Translation |
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| المؤلفون: | Fred Heffron, Joshua N. Adkins, Ernesto S. Nakayasu, Charles Ansong, Afshan S. Kidwai, Rudd C. Johnson, Christopher C. Overall, Eric D. Cambronne, Jie Li, Jason E. McDermott |
| المصدر: | Journal of Proteome Research. 14:1716-1726 |
| بيانات النشر: | American Chemical Society (ACS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Proteomics, Genetics, Salmonella, Gene Expression Profiling, Immunoblotting, Mutant, Sigma Factor, Gene Expression Regulation, Bacterial, General Chemistry, Biology, medicine.disease_cause, Biochemistry, Article, Transcriptome, Gene expression profiling, Sigma factor, Gene expression, Proteome, medicine, RNA Processing, Post-Transcriptional, Post-transcriptional regulation |
| الوصف: | The alternative sigma factor E (σ(E)) is critical for response to extracytoplasmic stress in Salmonella. Extensive studies have been conducted on σ(E)-regulated gene expression, particularly at the transcriptional level. Increasing evidence suggests however that σ(E) may indirectly participate in post-transcriptional regulation. In this study, we conducted sample-matched global proteomic and transcriptomic analyses to determine the level of regulation mediated by σ(E) in Salmonella. Samples were analyzed from wild-type and isogenic rpoE mutant Salmonella cultivated in three different conditions: nutrient-rich and conditions that mimic early and late intracellular infection. We found that 30% of the observed proteome was regulated by σ(E) combining all three conditions. In different growth conditions, σ(E) affected the expression of a broad spectrum of Salmonella proteins required for miscellaneous functions. Those involved in transport and binding, protein synthesis, and stress response were particularly highlighted. By comparing transcriptomic and proteomic data, we identified genes post-transcriptionally regulated by σ(E) and found that post-transcriptional regulation was responsible for a majority of changes observed in the σ(E)-regulated proteome. Further, comparison of transcriptomic and proteomic data from hfq mutant of Salmonella demonstrated that σ(E)-mediated post-transcriptional regulation was partially dependent on the RNA-binding protein Hfq. |
| تدمد: | 1535-3907 1535-3893 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5453eb3feb4081f20326bd396117c99dTest https://doi.org/10.1021/pr5010423Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5453eb3feb4081f20326bd396117c99d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15353907 15353893 |
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