Transfusion of 35‐day stored red blood cells does not result in increase of plasma non‐transferrin bound iron in human endotoxemia
| العنوان: | Transfusion of 35‐day stored red blood cells does not result in increase of plasma non‐transferrin bound iron in human endotoxemia |
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| المؤلفون: | Nicole P. Juffermans, Robin van Bruggen, Alexander P.J. Vlaar, Dirk de Korte, Anna L. Peters, Renoja K. Kunanayagam |
| المساهمون: | Graduate School, Intensive Care Medicine, Landsteiner Laboratory, Amsterdam Cardiovascular Sciences, ACS - Pulmonary hypertension & thrombosis, ACS - Microcirculation |
| المصدر: | Transfusion, 57(1), 53-59. Wiley-Blackwell |
| بيانات النشر: | Wiley, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Lipopolysaccharides, Male, medicine.medical_specialty, Time Factors, Blood transfusion, Adolescent, Iron, medicine.medical_treatment, Immunology, 030204 cardiovascular system & hematology, Blood Transfusion, Autologous, Hemoglobins, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, Lactate dehydrogenase, Humans, Immunology and Allergy, Medicine, chemistry.chemical_classification, Haptoglobins, L-Lactate Dehydrogenase, biology, business.industry, Transferrin saturation, Haptoglobin, Bilirubin, Hematology, medicine.disease, Endotoxemia, Hemolysis, Ferritin, Endocrinology, chemistry, Blood Preservation, Transferrin, Ferritins, biology.protein, Hemoglobin, Erythrocyte Transfusion, business, 030215 immunology |
| الوصف: | BACKGROUND Transfusion of a single unit of stored red blood cells (RBCs) has been hypothesized to induce supra-physiological levels of non-transferrin bound iron (NTBI), which may enhance inflammation and act as a nutrient for bacteria. We investigated the relation between RBC storage time and iron levels in a clinically relevant “two-hit” human transfusion model. STUDY DESIGN AND METHODS Eighteen healthy male volunteers (ages 18-35 years) were infused with 2 ng lipopolysaccharide (LPS)/kg to induce systemic inflammatory response syndrome. Two hours later, each participant received either 1 unit of 2-day stored (2D) autologous RBCs, 35-day stored (35D) autologous RBCs, or an equal volume of saline. Every 2 hours up to 8 hours after LPS infusion, hemoglobin, hemolysis parameters, and iron parameters, including NTBI, were measured. RESULTS Transfusion of both 2D and 35D RBCs caused increases in hemoglobin, plasma iron, and transferrin saturation; whereas levels remained stable in the saline group. Transfusion of 35D RBCs did not result in hemolysis nor did it lead to increased levels of NTBI compared with 2D RBCs or saline. LPS induced increases in ferritin, haptoglobin, bilirubin, and lactate dehydrogenase that were similar in all three groups. CONCLUSION We conclude that 35D autologous RBCs do not cause hemolysis or increased levels of NTBI during human endotoxemia. |
| تدمد: | 1537-2995 0041-1132 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53dd3b0edd58a9d83b4925c13633bb20Test https://doi.org/10.1111/trf.13849Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....53dd3b0edd58a9d83b4925c13633bb20 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15372995 00411132 |
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